Cellometer Auto T4 investigates dysfunction to pro-inflammatory cytokine toxicity and reactive oxygen species

The Institute of Clinical Biochemistry (Hanover, Germany) hypothesized that the pro-inflammatory cytokine environment seen in obese patients and those with obesity-related diabetes promotes the dysregulation of brown adipose tissues (BAT), which in turn intensifies diabetes progression. With a murine non-differentiated brown adipocyte cell line, researchers examined how exposure to pro-inflammatory cytokines impacted these cells. Cell density calculations were performed on the Cellometer Auto T4. The pro-inflammatory cytokines negatively impacted the cells’ viability, markedly increased reactive oxygen species production, and down regulated markers specific to BAT such as UCP-1 and β-Klotho. The scientists concluded that pro-inflammatory cytokines lead to BAT death [...]

By | 2016-03-01T10:00:13+00:00 March 1st, 2016|Categories: Cellometer User Publications|Tags: , , , |0 Comments

Cellometer assists in characterization of adipocytic extracellular vesticles

Adipocytes have been identified as a source of extracellular vesicles (EVs), which have, in turn, been connected to obesity-mediated cardiovascular disease. But these vesicles have not been well studied, particularly pre- and post-adipogenesis. Researchers at Cardiff University (UK) set out to characterize these EVs to assess their structure, composition, and function. 3T3-L1 cells were used to harvest EVs which were then characterized via electron microscopy, flow cytometry, and 2D chromatography. The Cellometer and Trypan Blue established cell counts throughout experimentation. Before adipogenesis, EVs display “classic” morphology, but the number of EVs rise just before adipogenesis and are enriched with signaling [...]

By | 2015-12-17T11:50:09+00:00 December 17th, 2015|Categories: Cellometer User Publications|Tags: , , , , |0 Comments