Assuring accurate cell viability is critical both pre- and post-thaw as the freezing process can alter cell characteristics.
Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample.
Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells
Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]
Obtain fast, accurate and consistent cell viability and concentration measurements of primary murine samples in <30 seconds using the Cellometer K2!
Bone marrow, cord blood, whole blood, and peripheral blood are routinely processed in many different laboratories. Whether for cryopreservation or for downstream isolation of specific nucleated cells, (such as stem cells, B-cells, or T-cells) accurately measuring cell concentration and viability is paramount to the overall success of the project. Many blood-based samples (whole blood, peripheral blood, bone marrow, PBMC, cord blood, etc…) may contain residual red blood cells even after RBC lysis. (See figure below) When samples are enumerated using manual counting, the presence of residual RBCs can lead to inaccurate cell concentration and viability readings. Nucleic acid dyes, acridine [...]