Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
If you are experiencing difficulty counting your PBMCs with Trypan Blue using a hemacytometer, we feel your pain! But did you know all that pain can be avoided? With the Cellometer Auto 2000 automated cell counter, counting peripheral blood mononuclear cells (PBMCs) and other complex primary samples is a breeze! Using dual fluorescence image-based cytometry and staining your samples with AO/PI (acridine orange and propidium iodide), the Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample. Here is what some of our happy customers counting PBMCs have [...]
Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells
Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]
Obtain fast, accurate and consistent cell viability and concentration measurements of primary murine samples in <30 seconds using the Cellometer K2! The use of mouse models is common across multiple fields of study and the samples acquired greatly vary in type and complexity, from splenocytes to tissue digests to tail vein blood. Tissue debris, cell debris, and red-blood cell contamination can lead to inconsistent cell counts from sample to sample when performing manual counting. Nexcelom's Cellometer instruments perform fluorescent based analysis using nucleic acid dyes Acridine Orange/ Propidium Iodide (AO/PI). In the dual color, AO/PI viability assay, only nucleated cells [...]
Bone marrow, cord blood, whole blood, and peripheral blood are routinely processed in many different laboratories. Whether for cryopreservation or for downstream isolation of specific nucleated cells, (such as stem cells, B-cells, or T-cells) accurately measuring cell concentration and viability is paramount to the overall success of the project. Many blood-based samples (whole blood, peripheral blood, bone marrow, PBMC, cord blood, etc…) may contain residual red blood cells even after RBC lysis. (See figure below) When samples are enumerated using manual counting, the presence of residual RBCs can lead to inaccurate cell concentration and viability readings. Nucleic acid dyes, acridine [...]