Watch the Demo: On an easy to use automated cell counter, the Spectrum Image Cytometer, you can perform simple cell-based assays!
Many factors and considerations must be optimized to develop the appropriate cell-based assays for gene therapy development.
Available on demand. In this informational webinar, Dr. Dmitry Kuksin, Nexcelom Senior Scientist, examines specific virology related assays such as plaque and foci formation, as well as antibody neutralization.
Webinar on Demand: Role of Aneuploidy and DNA Damage in the Onset of Human Solid Tumors – Lessons from Mouse Models of Chromosome Losses
Available on demand. During this webinar, Dr. Thomas will share his research in cancer biology related to DNA damage in the onset of solid tumors.
At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]
It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »
Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis [1, 2]. Originally, the system has been used for automated cell concentration and viability measurement using disposable counting slides with both bright-field (BR) and fluorescent (FL) imaging methods . The system has recently been demonstrated to perform fast and accurate cell population analysis comparable to the traditional flow cytometry method. Download our white paper »
Introduction Propofol, a widely used general anesthetic, has been shown to produce neurotoxicity in neonatal animal experiments [1-5]. The possible detrimental effects of anesthetics, particularly in developing brains, require further study, as recent studies suggest that general anesthesia given to children younger than four years of age can increase the chances of learning disabilities later in life . Here, researchers used embryonic neural stem cells to investigate the neurotoxic effects of propofol in vitro. Materials and Methods Cell culture and drug exposure Embryonic neural stem cells were collected from embryonic day 16 Sprague-Dawley rats. Resulting cells were cultured in 96-well [...]
c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision
Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas . The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action . The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]
Iron oxide-based nanoparticles conjugated NuBCP-9 increase MCF-7 apoptosis as assessed by Cellometer Vision CBA Image Cytometer
Introduction The B-cell lymphoma-2 (BCL-2) family of proteins includes many key regulators of cell survival, cell death, and apoptosis. The deregulation of certain BCL-2 proteins is one of the first steps towards tumorigenesis and subsequent therapeutic resistance [1-4]. Targeting these BCL-2 family members is therefore an important area of oncology research [2, 4, 5]. Here, a collaboration of researchers has explored the targeting of these BCL-2 proteins with a BCL-2 binding peptide known as NuBCP-9 (FSRSLHSLL), which induces a conformational change in BCL-2 that alters the protein’s function from cell protection to cell killing. Researchers paired NuBCP-9 with iron oxide-based [...]