Accurate counting and qualification of cells during the workflow of the single cell sequencing is essential to determine number of cells.
Cellometer automated cell counters have been facilitating CAR T manufacturing with accurate cell counting and analysis since 2010.
Tired of manual cell counting? Let's face it, manual cell counting is tedious. When your day is filled with culturing many cell lines at one time, that's hours of your day just to get the data you need. Not only that, the manual process can be riddled with judgment errors, miscounts, and user-to-user variability. I know what you’re thinking, "You're telling me my hours hunched over a microscope counting cells may not even be producing reliable data?!" Yup. You need a superhero. "So what are my options?" Well, what if there were a powerful superhero that could take a small [...]
How many brewers does it take to count yeast? This sounds like the beginning of a bad joke. In truth, cell counting is really simple but labor intensive & extremely time-consuming. With a Nexcelom Cellometer X2 – it only takes one click & 30 seconds per sample! Cellometer has earned a reputation as the “go to” cell counter for breweries seeking accurate yeast counts, viability & vitality. Just look at the map comparing the distribution of Nexcelom’s Cellometer automated yeast counters and the Top 50 breweries in the US. Data based on beer sales volume adapted from Brewery Association (BA) [...]
As the first automated cell counter ever manufactured, the Auto T4 set the standard with its pattern-recognition software and bright field imaging used to calculate cell count, concentration and viability with Trypan Blue.
...a neck massage! There's a new article out from Biocompare entitled "Cell Counts and More" - and we have to give joke credit to them. We think it's a pretty good one, and accurate, just like our instruments. One of our customers, Tiffany Sidwell, of UCLA Immunogenetics Center, even provided some feedback on the very topic last year. Tiffany shared: "Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom!" There are many, many benefits associated with [...]
https://youtu.be/4xUYjVIgOdQ If you're using a microscope and hemacytometer to count your cells and determine viability, you might have felt like Sheldon, when he vacations in Amy's lab and, among other things, helps out by counting the bacteria spores. Everything's going well until Amy returns to the lab, distracts Sheldon, and then he has to start over with his count. Fortunately for you, you don't have to be like Sheldon if you have an automated cell counter. We have bright field counters for cultured cells and Trypan Blue exclusion assays. We have dual fluorescent cell counters for messy, primary samples [...]
Researchers at the University of Manchester (UK) created a reference database of 1,800 quantified S. cerevisiae proteins via specific isotope labeling and mass spectrometry. This is the largest database of its kind created to date, and the goal was to obtain a better understanding of intracellular protein concentrations, important information for those involved in molecular systems biology. The Cellometer was used to maintain accurate cell counts throughout experimentation. This database can now serve as a standard for the yeast proteome in all research going forward. Read the full publication here. The Cellometer automated cell counters can provide many advantages in [...]
Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.
The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]