Cell Counting Archives | Nexcelom Bioscience

An Automated Cell Counting Superhero – All in the Palm of Your Hand
Gallery
An Automated Cell Counting Superhero – All in the Palm of Your Hand

Cellometer Product News, Latest News

An Automated Cell Counting Superhero – All in the Palm of Your Hand

Tired of manual cell counting? Let's face it, manual cell counting is tedious. When your day is filled with culturing many cell lines at one time, that's hours of your day just to get the data you need. Not only that, the manual process can be riddled with judgment errors, miscounts, and user-to-user variability. I know what you’re thinking, "You're telling me my hours hunched over a microscope counting cells may not even be producing reliable data?!" Yup. You need a superhero. "So what are my options?" Well, what if there were a powerful superhero that could take a small [...]

By Krishna Vallabhaneni, PhD| 2018-08-07T21:59:04+00:00 August 7th, 2018|Categories: Cellometer Product News, Latest News|Tags: automated cell counters, Automated cell counting, Cell Counting, Cellometer, Cellometer Auto T4, hemacytometer, manual cell counting|1 Comment

Cellometer on Tap – Yeast Cell Counting for Breweries
Gallery
Cellometer on Tap – Yeast Cell Counting for Breweries

Cellometer Product News, Latest News

Cellometer on Tap – Yeast Cell Counting for Breweries

How many brewers does it take to count yeast? This sounds like the beginning of a bad joke. In truth, cell counting is really simple but labor intensive & extremely time-consuming. With a Nexcelom Cellometer X2 – it only takes one click & 30 seconds per sample! Cellometer has earned a reputation as the “go to” cell counter for breweries seeking accurate yeast counts, viability & vitality. Just look at the map comparing the distribution of Nexcelom’s Cellometer automated yeast counters and the Top 50 breweries in the US. Data based on beer sales volume adapted from Brewery Association (BA) [...]

By Belen Belete-Gilbert| 2018-06-22T12:56:10+00:00 June 21st, 2018|Categories: Cellometer Product News, Latest News|Tags: Brewing, Cell Counting, Cell Viability, Cellometer, Cellometer X2, hemacytometer, Yeast, yeast viability|0 Comments

Don’t let clumpy samples prevent you from getting an accurate count!

Counting cells with a hemacytometer is a long, dull process on its own – but if you’re trying to count cells that are clumped together in your sample, it can become a real headache! Clumpy samples make it hard to discern the individual cells from one another, causing a high risk of human error and skewed cell counts. Your results can be extremely difficult to reproduce, as the cell count would likely vary from person to person counting the same clumpy sample, causing a lack of confidence in your data. Luckily, we have a solution. The Cellometer Auto T4 automated [...]

By Polly Mitchell| 2017-04-12T11:25:57+00:00 April 12th, 2017|Categories: Cellometer Application News|Tags: Automated cell counting, Bright Field, Cell Counting, cell lines, Cell Viability, Cellometer Auto T4, Clumpy Cells, hemacytometer|0 Comments

What do you need when you count cells manually with a microscope and hemacytometer?
Gallery
What do you need when you count cells manually with a microscope and hemacytometer?

General Discussions, Latest News

What do you need when you count cells manually with a microscope and hemacytometer?

...a neck massage! There's a new article out from Biocompare entitled "Cell Counts and More" - and we have to give joke credit to them. We think it's a pretty good one, and accurate, just like our instruments. One of our customers, Tiffany Sidwell, of UCLA Immunogenetics Center, even provided some feedback on the very topic last year. Tiffany shared: "Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom!" There are many, many benefits associated with [...]

By Polly Mitchell| 2018-06-15T15:36:30+00:00 April 5th, 2017|Categories: General Discussions, Latest News|Tags: accuracy, automated cell counters, Cell Counting, Cellometer, hemacytometer, microscope|0 Comments

Ever lose count when manually counting?

https://youtu.be/4xUYjVIgOdQ If you're using a microscope and hemacytometer to count your cells and determine viability, you might have felt like Sheldon, when he vacations in Amy's lab and, among other things, helps out by counting the bacteria spores. Everything's going well until Amy returns to the lab, distracts Sheldon, and then he has to start over with his count. Fortunately for you, you don't have to be like Sheldon if you have an automated cell counter. We have bright field counters for cultured cells and Trypan Blue exclusion assays. We have dual fluorescent cell counters for messy, primary samples [...]

By Polly Mitchell| 2017-01-19T14:21:50+00:00 January 19th, 2017|Categories: About Cells|Tags: Cell Counting, cell-based assays, hemacytometer, Viability|0 Comments

Cellometer assists in creation of reference database of 1,800 quantified yeast proteins

Researchers at the University of Manchester (UK) created a reference database of 1,800 quantified S. cerevisiae proteins via specific isotope labeling and mass spectrometry. This is the largest database of its kind created to date, and the goal was to obtain a better understanding of intracellular protein concentrations, important information for those involved in molecular systems biology. The Cellometer was used to maintain accurate cell counts throughout experimentation. This database can now serve as a standard for the yeast proteome in all research going forward. Read the full publication here. The Cellometer automated cell counters can provide many advantages in [...]

By Polly Mitchell| 2018-06-11T14:54:00+00:00 February 23rd, 2016|Categories: Cellometer User Publications|Tags: Automated cell counting, Cell Counting, Cellometer, Yeast|0 Comments

Cellometer helps to study microalgae metabolism for future industrial biotechnology applications

Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.

By Polly Mitchell| 2016-02-16T10:00:47+00:00 February 16th, 2016|Categories: Cellometer User Publications|Tags: Cell Counting, Cellometer, lipids, microalgae, starch|0 Comments

Celigo assists in optimizing CHO cells for biopharmaceutical production

The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]

By Polly Mitchell| 2018-06-11T14:52:12+00:00 February 11th, 2016|Categories: Celigo User Publications|Tags: 96-well plates, Celigo, Cell Counting, CHO, CHO cells, Hoechst, Novo Nordisk, Propidium Iodide|0 Comments

Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells

Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]

By Polly Mitchell| 2018-06-11T14:50:58+00:00 February 4th, 2016|Categories: Cellometer User Publications|Tags: AO/PI, Cell Counting, Cellometer Auto 2000, UCMSCs, umbilical cord cells|0 Comments

Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale

Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]

By Polly Mitchell| 2016-01-19T10:00:50+00:00 January 19th, 2016|Categories: Cellometer User Publications|Tags: Cell Counting, Cell Viability, Cellometer Auto 2000, hMSC, regenerative medicine|0 Comments