As the first automated cell counter ever manufactured, the Auto T4 set the standard with its pattern-recognition software and bright field imaging used to calculate cell count, concentration and viability with Trypan Blue.
...a neck massage! There's a new article out from Biocompare entitled "Cell Counts and More" - and we have to give joke credit to them. We think it's a pretty good one, and accurate, just like our instruments. One of our customers, Tiffany Sidwell, of UCLA Immunogenetics Center, even provided some feedback on the very topic last year. Tiffany shared: "Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom!" There are many, many benefits associated with [...]
https://youtu.be/4xUYjVIgOdQ If you're using a microscope and hemacytometer to count your cells and determine viability, you might have felt like Sheldon, when he vacations in Amy's lab and, among other things, helps out by counting the bacteria spores. Everything's going well until Amy returns to the lab, distracts Sheldon, and then he has to start over with his count. Fortunately for you, you don't have to be like Sheldon if you have an automated cell counter. We have bright field counters for cultured cells and Trypan Blue exclusion assays. We have dual fluorescent cell counters for messy, primary samples [...]
Researchers at the University of Manchester (UK) created a reference database of 1,800 quantified S. cerevisiae proteins via specific isotope labeling and mass spectrometry. This is the largest database of its kind created to date, and the goal was to obtain a better understanding of intracellular protein concentrations, important information for those involved in molecular systems biology. The Cellometer was used to maintain accurate cell counts throughout experimentation. This database can now serve as a standard for the yeast proteome in all research going forward. Read the full publication here. The Cellometer automated cell counters can provide many advantages in [...]
Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.
The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]
Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells
Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]
Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale
Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]
EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]
Oregon Health & Science University researchers continued their studies into the gender-related differences in peripheral immune system response after stroke that produce greater downstream damage in males than females, and what role the spleen may play in those differences. The goal of this work was to uncover which subset of immune system cells may contribute to those pathogenic effects after a stroke and how they vary by gender. Using primary leukocytes from transgenic mice and adoptive T-cell transfer, scientists injected specific cell types into splenectomized male and female mice 24 hours after stroke was induced. The Cellometer Auto T4 maintained [...]