Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines

South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]

Cellometer Vision participates in study to evaluate effects of lead nitrate on human leukemia cells

At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]

Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »

By | 2015-10-07T10:30:08+00:00 October 7th, 2015|Categories: Cellometer Application News|Tags: , , , , |0 Comments

c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision

Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas [1]. The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action [2]. The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]

By | 2015-01-29T09:00:15+00:00 January 29th, 2015|Categories: Cellometer User Publications|Tags: , , , |0 Comments

Cellometer Vision uncovers new mechanisms by which Type I interferons direct autophagy in vitro

Introduction A diverse family of cytokines, Type I interferons (IFNs), is a group of proteins responsible for antiviral, antiproliferative, and immunomodulatory functions [1, 2]. Treating cells with Type I IFNs induces autophagy, a cellular recycling process that is considered a key cell survival strategy [3]. It is well known that Type I IFNs work through the JAK-STAT pathway, but recent evidence suggests that the MAP kinase pathway can affect the expression of IFN-regulated genes [4, 5]. Researchers demonstrated that IFN-induced autophagy affects cell cycle as well as cellular proliferation in a variety of cell lines. Because autophagy plays a major [...]

Perform quick & easy cell based assays using Cellometer Image Cytometer

Using Cellometer Image Cytometer to Perform Consistent and Accurate Cell Cycle, Apoptosis, and GFP Analysis Cellometer K2 and Vision instruments are capable of not only accurately and quickly measuring the cell concentration and viability, but also have the capability to perform multiple cell-based assays. Below we highlight three such assays: Cell Cycle Apoptosis GFP Performing Cell Cycle Assay Analyze and compare the effects of different cell cycle pharmacological agents. Provides researchers with the ability to visually review and confirm counted cells. Use the provided FCS Express™ templates for quick and easy data analysis and presentation. Data acquired on the Cellometer [...]

By | 2014-04-24T14:22:54+00:00 April 24th, 2014|Categories: Cellometer Application News|Tags: , , , |0 Comments

Get Flow Results without Flow Cytometry

Cellometer Vision CBA Image Cytometry System Incorporation of FCS Express 4 Software (De Novo Software) into the Vision CBA Analysis System has enabled users to conduct image-based analysis of cell-based assays requiring enhanced gating for the most accurate, reliable, and reproducible results. Side-by-side comparison of results for apoptosis, cell cycle, mitochondrial potential, autophagy, ABC transporter assays, aggresome detection, CD4+ / CD8+ immunophenotyping, and other assays has shown Cellometer image-based analysis results to be comparable to flow cytometry. Flow core labs can utilize the Vision CBA instrument to pre-screen samples prior to sorting and flow cytometry analysis and to [...]

By | 2014-03-24T09:20:32+00:00 March 24th, 2014|Categories: Cellometer Product News|Tags: , , , , |0 Comments