Assuring accurate cell viability is critical both pre- and post-thaw as the freezing process can alter cell characteristics.
After the freeze-thaw cycle, when viability drops, trypan blue's cytotoxic effect can further damage cells when incubation is required, thus skewing the sample's viability.
How many brewers does it take to count yeast? This sounds like the beginning of a bad joke. In truth, cell counting is really simple but labor intensive & extremely time-consuming. With a Nexcelom Cellometer X2 – it only takes one click & 30 seconds per sample! Cellometer has earned a reputation as the “go to” cell counter for breweries seeking accurate yeast counts, viability & vitality. Just look at the map comparing the distribution of Nexcelom’s Cellometer automated yeast counters and the Top 50 breweries in the US. Data based on beer sales volume adapted from Brewery Association (BA) [...]
Celigo playing an important role in high throughput, high-resolution image acquisition for DNA damage detection. The Celigo S captures 16 images of each well that are then stitched into a single representation Open access article: Next generation high throughput DNA damage detection platform for genotoxic compound screening
As the first automated cell counter ever manufactured, the Auto T4 set the standard with its pattern-recognition software and bright field imaging used to calculate cell count, concentration and viability with Trypan Blue.
Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines
South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]
Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale
Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]
North Carolina State University investigated the immune response to the gram-negative bacteria Delftia spp., a water and soil species which has been identified in IV catheters. Delftia strain Cs1-4 and THP-1 cells (monocytes) were employed. Cellometer Auto T4 provided cell viability measurements with Trypan Blue to ensure a constant concentration of viable monocytes were present throughout experimentation. After Delftia stimulation of the monocytes, monocyte mortality rose, as did the production of TNF and pro-inflammatory proteins. This research confirms that Delftia does induce a pro-inflammatory reaction in monocytes and suggests that more research into the downstream effects of this bacteremia-associated strain [...]
How safe is your mouthwash? Researchers in Germany worked to answer that question. University of Leipzig (Germany) researchers investigated the cytotoxic effects of different antimicrobial mouth rinses (MRs) on gingival fibroblasts and epithelial cells. Although effective at killing infectious agents, most MRs have some cytotoxic impact on host tissue, which could delay the healing process the rinses are meant to enable. Human primary gingiva fibroblasts and human primary nasal epithelial cells were exposed to various MRs (Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET), and control) for varying lengths of time. The Cellometer Auto T4 analyzed cell [...]
Researchers at Cardiff University and First Affiliated Hospital of Fourth Military Medical University of Shaanxi, China investigated the mechanisms by which excessive adipogenesis and extracellular matrix production, namely in the form of hyaluronan acid (HA), expand orbital contents in Graves’ Orbitopathy (GO) patients. Currently, GO patients are treated with immunosuppressive and anti-inflammatory therapies that are largely ineffective. This study examined FOXOs and how their involvement in the PI3K pathway might be studied in order to uncover non-immunosuppressive therapy targets. Preadipocytes/fibroblasts (PFs) were used to study the outcomes when FOXO expression was enhanced and inhibited. The Cellometer Auto T4 and Trypan [...]