French Institute for Health and Medical Research and the University of Lyon use Cellometer Auto T4 for Trypan Blue Viability

Here’s another great example of how researchers across the world are using the Cellometer instruments before performing their downstream assays: Researchers at the French Institute for Health and Medical Research and the University of Lyon investigated the effects of ultrasound on sonoporation (increasing a cell’s permeability in order to transfer molecules or genetic material into the cells). Using adherent HT-29 cells, as well as the Cellometer Auto T4 and trypan blue, researchers evaluated the cells’ viability 24 hours prior to sonication studies. Varying the acoustic intensities and cavitation regulation allowed them to optimize sonoporation efficiency, regardless of ambient temperature. The [...]

By |2015-11-06T10:16:04+00:00November 6th, 2015|Categories: Cellometer User Publications|Tags: , , |0 Comments

Rowan Univ Researchers using Cellometer Vision for PI Viability, Caspase-3 activation and ROS

The Cellometer instruments have a wide range of functionality – this publication demonstrates how an instrument such as the Cellometer Vision can be used for more than just viability and can perform many cell-based assays. Fructose differs from glucose in that it is not regulated by a negative feedback loop and can thereby negatively impact intracellular energy stores. As the incidence of diabetes and obesity have risen in this country, so has the intake of fructose, making fructose’s effects on pancreatic cells important to understand. Here, researchers at Rowan University revealed that fructose sensitizes pancreatic beta cells to TNF-a-induced toxicity. [...]

UNM College of Pharmacy uses Cellometer to perform TB and AO/PI Viability

Here's a great example of how the Cellometer is being used by researchers in toxicology. The University of New Mexico College of Pharmacy investigated the mechanisms by which environmentally-relevant concentrations of inorganic arsenic contribute to genotoxicity. Inorganic arsenic is present in the air, food, and drinking water in many countries and has been linked to various cancers, skin lesions, and immune system disorders, although the exact mechanisms are not yet clear. To study arsenic’s genotoxic mechanisms, the researchers employed Nexcelom’s Cellometer to assess cell number and viability of primary mouse thymus cells and the murine thymus pre-T cell line, D1 [...]

By |2015-10-19T14:42:41+00:00October 19th, 2015|Categories: Cellometer User Publications|Tags: , , , |0 Comments