Effective long-term cryopreservation of human mesenchymal stem cells is an essential component of regenerative medicine and tissue engineering applications.
This webinar takes a look at primary cell analysis and the difficulties of counting with traditional methods and presents an alternative of fluorescent counting.
This video shows how to use two-color fluorescence to count live and dead primary cells. PBMCs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds.
This video is an overview of the assays that can be done using the Cellometer Auto 2000. The combination of bright filed plus dual fluorescence are used to measure concentration and viability of stem cells, primary cells and cell lines in messy samples using acridine orange and propidium iodide.
This video demonstration features the Cellometer Auto X4 to measure algae cell concentration from a pond water sample.
Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample.
Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells
Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]
Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale
Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]
Join us for our next Cellometer User Training Webinar on Friday November 14th at 11am: How to Measure Concentration and Viability of Primary Cell Samples Using the Cellometer Auto 2000 This informational webinar will cover: A brief introduction to the Cellometer Auto 2000 How to measure concentration and viability of primary cells Example counted images of tested primary cells Which viability assay is right for me? Register here: https://www3.gotomeeting.com/register/229013454 All webinars are recorded. If you know you cannot attend but are interested in the topic, please register. After the webinar is concluded, you will receive an email with a link to [...]
The International Society for the Study of Xenobiotics is hosting their annual meeting October 19-23, 2014 at the Hilton San Francisco in San Francisco, CA. Nexcelom Bioscience will be exhibiting at booth # 314. We have 3 instruments that are specifically relevant for this field: Cellometer Auto 2000, Cellometer K2 and Celigo S. If you're attending ISSX 2014, stop by the booth, talk to Justin or Tim, and learn about how these instruments can benefit you and your lab! If you aren't able to make it out to ISSX this year, but would like more information about any of these [...]