This video demonstrates how to improve cell counting speed and accuracy using the Cellometer Auto T4 bright field automated cell counter.
Tired of manual cell counting? Let's face it, manual cell counting is tedious. When your day is filled with culturing many cell lines at one time, that's hours of your day just to get the data you need. Not only that, the manual process can be riddled with judgment errors, miscounts, and user-to-user variability. I know what you’re thinking, "You're telling me my hours hunched over a microscope counting cells may not even be producing reliable data?!" Yup. You need a superhero. "So what are my options?" Well, what if there were a powerful superhero that could take a small [...]
As the first automated cell counter ever manufactured, the Auto T4 set the standard with its pattern-recognition software and bright field imaging used to calculate cell count, concentration and viability with Trypan Blue.
Cellometer Auto T4 investigates dysfunction to pro-inflammatory cytokine toxicity and reactive oxygen species
The Institute of Clinical Biochemistry (Hanover, Germany) hypothesized that the pro-inflammatory cytokine environment seen in obese patients and those with obesity-related diabetes promotes the dysregulation of brown adipose tissues (BAT), which in turn intensifies diabetes progression. With a murine non-differentiated brown adipocyte cell line, researchers examined how exposure to pro-inflammatory cytokines impacted these cells. Cell density calculations were performed on the Cellometer Auto T4. The pro-inflammatory cytokines negatively impacted the cells’ viability, markedly increased reactive oxygen species production, and down-regulated markers specific to BAT such as UCP-1 and β-Klotho. The scientists concluded that pro-inflammatory cytokines lead to BAT death and [...]
Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines
South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]
North Carolina State University investigated the immune response to the gram-negative bacteria Delftia spp., a water and soil species which has been identified in IV catheters. Delftia strain Cs1-4 and THP-1 cells (monocytes) were employed. Cellometer Auto T4 provided cell viability measurements with Trypan Blue to ensure a constant concentration of viable monocytes were present throughout experimentation. After Delftia stimulation of the monocytes, monocyte mortality rose, as did the production of TNF and pro-inflammatory proteins. This research confirms that Delftia does induce a pro-inflammatory reaction in monocytes and suggests that more research into the downstream effects of this bacteremia-associated strain [...]
Oregon Health & Science University researchers continued their studies into the gender-related differences in peripheral immune system response after stroke that produce greater downstream damage in males than females, and what role the spleen may play in those differences. The goal of this work was to uncover which subset of immune system cells may contribute to those pathogenic effects after a stroke and how they vary by gender. Using primary leukocytes from transgenic mice and adoptive T-cell transfer, scientists injected specific cell types into splenectomized male and female mice 24 hours after stroke was induced. The Cellometer Auto T4 maintained [...]
How safe is your mouthwash? Researchers in Germany worked to answer that question. University of Leipzig (Germany) researchers investigated the cytotoxic effects of different antimicrobial mouth rinses (MRs) on gingival fibroblasts and epithelial cells. Although effective at killing infectious agents, most MRs have some cytotoxic impact on host tissue, which could delay the healing process the rinses are meant to enable. Human primary gingiva fibroblasts and human primary nasal epithelial cells were exposed to various MRs (Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET), and control) for varying lengths of time. The Cellometer Auto T4 analyzed cell [...]
Researchers at Cardiff University and First Affiliated Hospital of the Fourth Military Medical University of Shaanxi, China investigated the mechanisms by which excessive adipogenesis and extracellular matrix production, namely in the form of hyaluronan acid (HA), expand orbital contents in Graves’ Orbitopathy (GO) patients. Currently, GO patients are treated with immunosuppressive and anti-inflammatory therapies that are largely ineffective. This study examined FOXOs and how their involvement in the PI3K pathway might be studied in order to uncover non-immunosuppressive therapy targets. Preadipocytes/fibroblasts (PFs) were used to study the outcomes when FOXO expression was enhanced and inhibited. The Cellometer Auto T4 and [...]
French Institute for Health and Medical Research and the University of Lyon use Cellometer Auto T4 for Trypan Blue Viability
Here’s another great example of how researchers across the world are using the Cellometer instruments before performing their downstream assays: Researchers at the French Institute for Health and Medical Research and the University of Lyon investigated the effects of ultrasound on sonoporation (increasing a cell’s permeability in order to transfer molecules or genetic material into the cells). Using adherent HT-29 cells, as well as the Cellometer Auto T4 and trypan blue, researchers evaluated the cells’ viability 24 hours prior to sonication studies. Varying the acoustic intensities and cavitation regulation allowed them to optimize sonoporation efficiency, regardless of ambient temperature. The [...]