This webinar takes a look at primary cell analysis and the difficulties of counting with traditional methods and presents an alternative of fluorescent counting.
This webinar takes a look cell cycle. How is it currently examined? Is there a better way? What if cell cycle assays could be performed in a high throughput method?
This video will show you how to perform apoptosis analysis based on changes in mitochondrial membrane potential with only 20µl of sample.
This video will show you how to simplify apoptosis experiments with image-based analysis. Colorized data plots easily identify apoptotic and necrotic cells.
This video will show you how to perform cell cycle analysis with only 20µl of sample. See how image-based analysis simplifies cell-based assays.
This video will show you how to perform a fast, simple, automated method of GFP analysis that requires only 20 µl of cell sample.
This video will show you how to perform a kinetic apoptosis assay on the Celigo image cytometer using caspase 3/7 detection reagent and suspension Jurkat cells.
If you rely on a flow cytometer in a core lab or a central facilities lab, have you ever wondered if there was a better way? Have you ever wished to have full control over your experiments, rather than hand your sample over to a technician? There was an interesting article written recently about the needs of researchers. Have you seen this? Those interviewed for the article suggest that there are some main factors for consideration: The instrument size needs a smaller footprint than existing products offer The price of the instrument should be under $100,000 Software ease-of-use to reduce [...]
Introduction Propofol, a widely used general anesthetic, has been shown to produce neurotoxicity in neonatal animal experiments [1-5]. The possible detrimental effects of anesthetics, particularly in developing brains, require further study, as recent studies suggest that general anesthesia given to children younger than four years of age can increase the chances of learning disabilities later in life . Here, researchers used embryonic neural stem cells to investigate the neurotoxic effects of propofol in vitro. Materials and Methods Cell culture and drug exposure Embryonic neural stem cells were collected from embryonic day 16 Sprague-Dawley rats. Resulting cells were cultured in 96-well [...]
Cellometer Vision CBA image cytometry reveals the utility of the immunocompetent mouse model “Glowing Head” in assessing preclinical anti-tumor therapies
Introduction Preclinical models to evaluate therapeutic efficacy in the area of oncology have proven to be a challenge. The standard of preclinical assessment as always been immunosuppressed mice, but that model has not reliably predicted clinical efficacy . Immunocompetent genetically engineered mice with tumor allograft models seem more promising, and yet reporter tags such as GFP and luciferase are targeted by competent immune systems, which may interfere with tumor growth and the response to therapeutics. Here, a collaborative group of researchers has created a reporter-tolerized genetically engineered mouse model, dubbed “Glowing Head” (GH), by targeting a luciferase-GFP reporter into the [...]