Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision Automated cell counting methods can monitor yeast concentration and viability throughout fermentation to ascertain cell health and the amount of yeast to be pitched or repitched, all of which contributes to the quality and flavor consistency of the final product [1,2,3]. Analyzing physiological and metabolic characteristics of the yeast cells permits operators to efficiently monitor yeast viability and vitality for quality control purposes, which impacts long-term storage and other physiological stresses.     Download our white [...]

A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens [1]. A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α [2]. The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets [3]. Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy.     Download our white [...]

By | 2016-03-02T10:00:54+00:00 March 2nd, 2016|Categories: Cellometer Application News|Tags: , , |0 Comments

Cellometer Vision helps undergraduates explore immunophenotyping in mouse bone marrow stem cells

A collaboration between Merrimack College (North Andover, MA) and Nexcelom Bioscience LLC used a Cellometer Vision in an undergraduate immunology classroom to explore differentiation, activation, cell surface marker expression and cytokine production in mouse bone marrow stem cells. The Cellometer Vision allowed the students to visualize and analyze their cells for various surface markers before designing experiments to explore the activity of natural anti-inflammatory compounds on TNF-alpha production. Read the full publication here.  More than 50% of Nexcelom employees hold advanced degrees - so it's no surprise that we love collaborations with other scientists and institutions. Many of our academic [...]

Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation These studies reveal that over a time course analysis in standard cell culture, TB exclusion reports more viability in comparison to the fluorescence-based tools, although the studies did not speculate as to the reasoning behind the differences noted (Jones and Senft 1985; Mascotti et al. 2000). In this work, we perform a head-to-head comparison between cell viability as determined by TB and that determined by AO/PI using an automated image-based cytometer, the Cellometer. Image-based cytometry allowed us to examine the [...]

Cellometer Vision is used to characterize fungi suitable for pest management

Entomopathogenic fungi (ENPF)– those fungal species that use insect hosts to propagate – are of note in India due to their potential utility in pest management. The Indian Institute of Technology (New Delhi, India) obtained soil samples and isolated ENPF with the intention of studying how these types of fungi might be used to control the house fly, Musca domestica, an insect that causes many health problems in humans and animals in that country. The samples were then characterized molecularly and tested for pathogenicity against M. domestica. The Cellometer Vision was used to accurately maintain spore counts. Through this work, [...]

Cellometer Vision provides new method for measuring cytotoxic potential in Natural Killer Cells

The MD Anderson Cancer Center worked in collaboration with Nexcelom to create a new method by which to measure the cytotoxic potential of natural killer (NK) cells. The traditional non-radioactive method, calcein release, is subject to variation, with differing dynamic ranges depending on tumor type. The Cellometer Vision and calcein, in combination with K562, 721.221, and Jurkat cells, were used to develop a novel, image cytometry-based assay to ascertain NK cytotoxicity. Using fluorescent intensity gating to ignore dimmer cells and apoptotic bodies, image cytometry provided a way to measure tumor cell lysis in a specific manner with the same experimental [...]

Rowan Univ Researchers using Cellometer Vision for PI Viability, Caspase-3 activation and ROS

The Cellometer instruments have a wide range of functionality – this publication demonstrates how an instrument such as the Cellometer Vision can be used for more than just viability and can perform many cell-based assays. Fructose differs from glucose in that it is not regulated by a negative feedback loop and can thereby negatively impact intracellular energy stores. As the incidence of diabetes and obesity have risen in this country, so has the intake of fructose, making fructose’s effects on pancreatic cells important to understand. Here, researchers at Rowan University revealed that fructose sensitizes pancreatic beta cells to TNF-a-induced toxicity. [...]

By | 2015-11-04T10:15:35+00:00 November 4th, 2015|Categories: Cellometer User Publications|Tags: , , |0 Comments

c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision

Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas [1]. The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action [2]. The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]

By | 2015-01-29T09:00:15+00:00 January 29th, 2015|Categories: Cellometer User Publications|Tags: , , , |0 Comments

Cellometer Vision uncovers new mechanisms by which Type I interferons direct autophagy in vitro

Introduction A diverse family of cytokines, Type I interferons (IFNs), is a group of proteins responsible for antiviral, antiproliferative, and immunomodulatory functions [1, 2]. Treating cells with Type I IFNs induces autophagy, a cellular recycling process that is considered a key cell survival strategy [3]. It is well known that Type I IFNs work through the JAK-STAT pathway, but recent evidence suggests that the MAP kinase pathway can affect the expression of IFN-regulated genes [4, 5]. Researchers demonstrated that IFN-induced autophagy affects cell cycle as well as cellular proliferation in a variety of cell lines. Because autophagy plays a major [...]

Cellometer Training Webinars available on YouTube!

Nexcelom hosts free, live monthly training webinars for our Cellometer instruments. Our webinars cover a variety of instruments, cell types and applications. We have taken our recorded webinars and added them to our YouTube channel! We've created a playlist entitled "Cellometer Training Webinars" and the list will continue to grow as we release more. This is a great resource for you and new lab team members to get additional training on your Cellometer instrument. We host new training webinars each month, so revisit our YouTube channel from time to time to see new content as it's produced and added.