Tired of manual cell counting? Let's face it, manual cell counting is tedious. When your day is filled with culturing many cell lines at one time, that's hours of your day just to get the data you need. Not only that, the manual process can be riddled with judgment errors, miscounts, and user-to-user variability. I know what you’re thinking, "You're telling me my hours hunched over a microscope counting cells may not even be producing reliable data?!" Yup. You need a superhero. "So what are my options?" Well, what if there were a powerful superhero that could take a small [...]
How many brewers does it take to count yeast? This sounds like the beginning of a bad joke. In truth, cell counting is really simple but labor intensive & extremely time-consuming. With a Nexcelom Cellometer X2 – it only takes one click & 30 seconds per sample! Cellometer has earned a reputation as the “go to” cell counter for breweries seeking accurate yeast counts, viability & vitality. Just look at the map comparing the distribution of Nexcelom’s Cellometer automated yeast counters and the Top 50 breweries in the US. Data based on beer sales volume adapted from Brewery Association (BA) [...]
...a neck massage! There's a new article out from Biocompare entitled "Cell Counts and More" - and we have to give joke credit to them. We think it's a pretty good one, and accurate, just like our instruments. One of our customers, Tiffany Sidwell, of UCLA Immunogenetics Center, even provided some feedback on the very topic last year. Tiffany shared: "Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom!" There are many, many benefits associated with [...]
Our founder, Dr. Jean Qiu, developed the Cellometer Auto T4 in 2005 after receiving a request for such an instrument from a customer at NIH, and we officially launched it at AACR 2006. As we come up on the 10 year anniversary of the original launch of our first automated cell counter it got us thinking. When we created the first automated cell counter, we created the market for such devices and we were alone in the equipment space for 2-3 years before any other company came out with competitive products. (Imitation is the highest form of flattery, right?) It was [...]
The University of Kentucky investigated progesterone receptor membrane component 1 (PGRMC1), an often upregulated component in thyroid, breast, colon and lung tumors. PGRMC1 has been associated with drug resistance and is thought of as an indicator of prognosis. The researchers employed a variety of cell types to represent head and neck cancers, as well as oral, lung and ovarian cancers. These cells were exposed to PGRMC1 inhibitors. The Cellometer performed cell counts with Trypan Blue. The PGRMC1 inhibitors successfully prompted cancer stem cell death even when other anti-cancer agents did not. The researchers suggest using PGRMC1 as a cancer stem [...]
The Max Planck Institute of Molecular Plant Physiology (Germany) investigated the evolutionarily-conserved proteins REIL 1 and 2 in A. thaliana and yeast cells. Research suggests these proteins are involved in the eukaryotic ribosomal 60S subunit. Here, investigators studied mutated REIL proteins in different species. The Cellometer Auto M10 analyzed cell size and concentration. The group discovered that these proteins are necessary to allow A. thaliana to grow in lower temperatures. Read the full publication here.
Researchers at the University of Manchester (UK) created a reference database of 1,800 quantified S. cerevisiae proteins via specific isotope labeling and mass spectrometry. This is the largest database of its kind created to date, and the goal was to obtain a better understanding of intracellular protein concentrations, important information for those involved in molecular systems biology. The Cellometer was used to maintain accurate cell counts throughout experimentation. This database can now serve as a standard for the yeast proteome in all research going forward. Read the full publication here. The Cellometer automated cell counters can provide many advantages in [...]
Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.
EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]
At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]