Cellometer Vision uncovers new mechanisms by which Type I interferons direct autophagy in vitro

Introduction A diverse family of cytokines, Type I interferons (IFNs), is a group of proteins responsible for antiviral, antiproliferative, and immunomodulatory functions [1, 2]. Treating cells with Type I IFNs induces autophagy, a cellular recycling process that is considered a key cell survival strategy [3]. It is well known that Type I IFNs work through the JAK-STAT pathway, but recent evidence suggests that the MAP kinase pathway can affect the expression of IFN-regulated genes [4, 5]. Researchers demonstrated that IFN-induced autophagy affects cell cycle as well as cellular proliferation in a variety of cell lines. Because autophagy plays a major [...]

Using Cellometer Vision and Cyto-ID Stain for Autophagy Detection in Living Cells

It's White Paper Wednesday! This month's featured white paper: Using Cellometer Vision and Cyto-ID Stain for Autophagy Detection in Living Cells Our understanding of autophagy has expanded tremendously in recent years, largely due to the identification of the many genes involved in the process, and the use of GFP-LC3 fusion proteins to visually monitor autophagosomes and autophagic activity both biochemically and microscopically [1, 2]. Recently, a novel fluorescent probe, Cyto-ID ® Green autophagy dye, has been developed to facilitate the investigation of the autophagic process [3-5]. In this study, a novel method was performed using the Cellometer image cytometry in combination with Cyto-ID Green autophagy dye for [...]

By | 2018-06-20T20:17:12+00:00 September 3rd, 2014|Categories: Cellometer Application News|Tags: , , |0 Comments