View these simple step-by-step instructions for counting cells using a hemoctyometer and microscope. Includes the formula for calculating the dilution factor.
Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
The use of modern automation has largely eliminated many of the hemocytometer's sources of error, increasing the accuracy and efficiency of cell counting today.
It's White Paper Wednesday! Read our featured white paper:The Historical Development of the Hemacytometer The hemacytometer has been an essential tool for hematologists, medical practitioners, and biologists for over a century. Depending on where it is being used, the word has multiple spellings such as hemacytometer, hemocytometer, haemacytometer, or haemocytometer, but for consistency purposes the word “hemacytometer” will be used in this review. The prefix “hema”, “hemo”, “haema”, or “haemo” means blood, while “cytometer” meant a device to measure cells. The device was initially used by medical practitioners to analyze patient blood samples, which was the initial spark that created [...]
It’s White Paper Wednesday! This month’s featured white paper: Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation The hemacytometer persists as the gold standard for laboratory cell counting. First utilized in 18th century France as a means to analyze patient blood samples, the hemacytometer has gone through a series of major developments over the past hundreds of years, creating a modern instrument that is more accurate and easier to use than its predecessors. The hemacytometer remains an integral part of all cell-based research, and yet sources of error inherent in its design and utilization persist. Those sources [...]