There is no single “one size fits all” cell-based assay however 3D cell cultures have been shown to be more physiologically relevant in oncology, immune oncology and other fields.
During this webinar, Dr. Suzanne Riches highlights a variety of approaches for conducting virology assays in a high-throughput format.
Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.
Celigo Image Cytometer provides a fast, effective, and cell stress-free technique to count cells plated onto uncoated or coated culture plates.
How to use Celigo image cytometry to normalize Seahorse™ XF data and eliminate time-consuming protein-based process.
Come visit us at booth #413 Merck Technology Symposium 2018 September 5-6, 2018 Ocean Place Resort Long Branch, NJ Posters being presented: High-throughput foci counting of viral titer and antibody neutralization assays using the Celigo Image Cytometer for developing a novel cross-reactive influenza vaccine Long-term time-course monitoring of NK cell-mediated ADCC using the Celigo Image Cytometer A high-throughput 3D tumor spheroid screening method for drug discovery using imaging cytometry
Cellometer automated cell counters have been facilitating CAR T manufacturing with accurate cell counting and analysis since 2010.
The FDA identifies clonality as one of the most crucial steps in guaranteeing cell line quality and safety. The Celigo Image Cytometer makes single cell sorting easy.
The Danish Cancer Society Research Center recently published a study furthering their analysis of homologous recombination DNA repair machinery. The group previously reported on a growth factor, PSIP1, that enables DNA end resection. With GFP-transfected U2OS cells, the group investigated a structurally similar protein, hepatoma-derived growth factor-related protein 2 (HDGFRP2). The Celigo analyzed cell number and viability via fluorescent markers. The group reports that HDGRFP2 may help to repair silent genes that have been impaired or active genes inhibited by DNA damage. Read the full publication here.
At the Canary Center at Stanford for Early Cancer Detection, investigators studied how AshwaMAX (a steroidal lactone from a winter cherry plant, Withania somnifera, extract) might work as an oral treatment for those with the highly aggressive cancer glioblastoma multiforme (GBM). A heterogeneous disease, non-specific therapies for GBM have proven largely ineffective. Two patient-derived GBM lines (GBM2, GBM39) and one GBM cell line were cultured to create neurospheres that were then exposed to various concentrations of AshwaMAX. Celigo measured cell proliferation and cell death via Trypan Blue staining. AshwaMAX inhibited the neurospheres at nanomolar concentrations. After additional work in vivo, [...]