/Tag: jurkat cells

Cellometer K2 aids in Anti-Cancer Immunity Research

The MD Anderson Cancer Center investigated how a patient’s anti-cancer T cells might be better protected from the cytotoxic effects of the anti-thymidylate drugs (AThys; such as methotrexate) used to treat lung, breast, colon, and pancreas cancers. Although AThys successfully attack cancer cells, the drugs also reduce a patient’s own anti-cancer T cell population, which plays an important role in helping the patient overcome the disease. Using Jurkat, AaPC cells, and mutated human proteins called muteins, researchers were able to manufacture T cells that were resistant to cytotoxic levels of AThys. The Cellometer K2 and Trypan Blue were used to [...]

Cellometer Vision provides new method for measuring cytotoxic potential in Natural Killer Cells

The MD Anderson Cancer Center worked in collaboration with Nexcelom to create a new method by which to measure the cytotoxic potential of natural killer (NK) cells. The traditional non-radioactive method, calcein release, is subject to variation, with differing dynamic ranges depending on tumor type. The Cellometer Vision and calcein, in combination with K562, 721.221, and Jurkat cells, were used to develop a novel, image cytometry-based assay to ascertain NK cytotoxicity. Using fluorescent intensity gating to ignore dimmer cells and apoptotic bodies, image cytometry provided a way to measure tumor cell lysis in a specific manner with the same experimental [...]