ELISpot is an assay for the detection and enumeration of cytokine secreting cells. Accurate cell counting is important for ELISpot.
Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
Obtain fast, accurate and consistent cell viability and concentration measurements of primary murine samples in
Bone marrow, cord blood, whole blood, and peripheral blood are routinely processed in many different laboratories. Whether for cryopreservation or for downstream isolation of specific nucleated cells, (such as stem cells, B-cells, or T-cells) accurately measuring cell concentration and viability is paramount to the overall success of the project. Many blood-based samples (whole blood, peripheral blood, bone marrow, PBMC, cord blood, etc…) may contain residual red blood cells even after RBC lysis. (See figure below) When samples are enumerated using manual counting, the presence of residual RBCs can lead to inaccurate cell concentration and viability readings. Nucleic acid dyes, acridine [...]