ELISpot is an assay for the detection and enumeration of cytokine secreting cells. Accurate cell counting is important for ELISpot.
Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample.
It’s White Paper Wednesday! This month’s featured white paper: Accurately Count PBMC and Measure Viability in Presence of Residual RBC In this work, we have developed an image cytometry method for detecting and monitoring the cell expansion and differentiation of articular chondrocytes in primary culture. First, the feasibility of utilizing image cytometry for detection of fluorescent is shown by comparing measured fluorescent positive cell populations to flow cytometry. Next, articular chondrocyte cultures were established in multi-well plates from either single or Cyan/eGFP double reporter mouse lines and grown for 20 days to test the utility of the fluorescence-based image cytometry system. [...]