/Tag: Propidium Iodide

Celigo assists in optimizing CHO cells for biopharmaceutical production

The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]

Cellometer Vision participates in study to evaluate effects of lead nitrate on human leukemia cells

At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]

Cellometer investigates new treatment paradigm for advanced endometrial cancer

University of North Carolina researchers investigated different techniques for inhibiting the catalytic activity of protein hTERT – a marker of advanced stage endometrial cancer. Endometrial cancer cell lines ECC-1 and Ishikawa were exposed to either siRNA or a small molecule pharmacological inhibitor BIBR1532, in addition to the drug paclitaxel, to see whether inhibiting hTERT provided additional efficacy against these cancer cells. The Cellometer, in combination with propidium iodide and Annexin-V FITC, calculated apoptosis in the various treatment conditions. The hTERT inhibition plus paclitaxel did prove synergistic, reducing cell growth and invasion more than paclitaxel alone. Furthermore, BIBR1532 antagonized cell invasion [...]

Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation Determining cell viability is a vital component in many biological experiments that range from standard cell culture to the investigation of pharmacological agents on tumor cells. One of the earliest and most common methods for measuring cell viability is the trypan blue (TB) exclusion assay [1, 2]. Over the last two decades, there have been various publications on comparing TB exclusion and fluorescence-based cellular viability assays [1, 3-5]. Previous results have shown that in a time-course measurement, TB exclusion [...]