/Tag: Stem Cell

Cellometer helps with culturing optimization necessary for future cell-based therapies

EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]

Cellometer Vision tracks apoptosis in embryonic neural stem cells after propofol exposure

Introduction Propofol, a widely used general anesthetic, has been shown to produce neurotoxicity in neonatal animal experiments [1-5]. The possible detrimental effects of anesthetics, particularly in developing brains, require further study, as recent studies suggest that general anesthesia given to children younger than four years of age can increase the chances of learning disabilities later in life [6]. Here, researchers used embryonic neural stem cells to investigate the neurotoxic effects of propofol in vitro. Materials and Methods Cell culture and drug exposure Embryonic neural stem cells were collected from embryonic day 16 Sprague-Dawley rats. Resulting cells were cultured in 96-well [...]