/Tag: Trypan Blue vs AOPI

Is your trypan blue rupturing dead or dying immune cells leading to an over-estimation of cell viability?

It is important for biologists to select the most accurate cell counting method that is fit-for-purpose, especially for cellular therapy products such as CAR T, where cell number and viability significantly affect treatment efficacy.

Addressing Primary Cell Counting Concerns

Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.

Why Are You Using Trypan Blue to Determine Viability Post-Thaw?

After the freeze-thaw cycle, when viability drops, trypan blue's cytotoxic effect can further damage cells when incubation is required, thus skewing the sample's viability.

By |2018-11-05T20:09:50+00:00November 5th, 2018|Categories: Latest News|Tags: , , , |0 Comments