Webinar on Demand: Accurate and consistent cell counting methods for development and manufacturing of CAR-T under GMP/GLP conditions.
Is your trypan blue rupturing dead or dying immune cells leading to an over-estimation of cell viability?
It is important to select the most accurate cell counting method that is fit-for-purpose where cell number and viability significantly affect treatment efficacy.
Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
ELISpot is an assay for the detection and enumeration of cytokine secreting cells. Accurate cell counting is important for ELISpot.
Did your lab’s grant fall through? Funding not an option? We get it – money’s tight right now. In Nexcelom’s long line of automated cell counters, there is one instrument designed specifically with your budget in mind – the Cellometer Mini! As the lowest-cost cell counter on the market, it utilizes bright field imaging, and is perfect for any lab looking to achieve basic cell count, concentration and Trypan Blue viability of cultured cell lines and purified primary samples. It’s affordable, accurate, and small enough to fit in your purse or backpack! Need a little convincing? Don’t just take our word [...]
The University of Kentucky investigated progesterone receptor membrane component 1 (PGRMC1), an often upregulated component in thyroid, breast, colon and lung tumors. PGRMC1 has been associated with drug resistance and is thought of as an indicator of prognosis. The researchers employed a variety of cell types to represent head and neck cancers, as well as oral, lung and ovarian cancers. These cells were exposed to PGRMC1 inhibitors. The Cellometer performed cell counts with Trypan Blue. The PGRMC1 inhibitors successfully prompted cancer stem cell death even when other anti-cancer agents did not. The researchers suggest using PGRMC1 as a cancer stem [...]
Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines
South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]
North Carolina State University investigated the immune response to the gram-negative bacteria Delftia spp., a water and soil species which has been identified in IV catheters. Delftia strain Cs1-4 and THP-1 cells (monocytes) were employed. Cellometer Auto T4 provided cell viability measurements with Trypan Blue to ensure a constant concentration of viable monocytes were present throughout experimentation. After Delftia stimulation of the monocytes, monocyte mortality rose, as did the production of TNF and pro-inflammatory proteins. This research confirms that Delftia does induce a pro-inflammatory reaction in monocytes and suggests that more research into the downstream effects of this bacteremia-associated strain [...]
At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]
Adipocytes have been identified as a source of extracellular vesicles (EVs), which have, in turn, been connected to obesity-mediated cardiovascular disease. But these vesicles have not been well studied, particularly pre- and post-adipogenesis. Researchers at Cardiff University (UK) set out to characterize these EVs to assess their structure, composition, and function. 3T3-L1 cells were used to harvest EVs which were then characterized via electron microscopy, flow cytometry, and 2D chromatography. The Cellometer and Trypan Blue established cell counts throughout experimentation. Before adipogenesis, EVs display “classic” morphology, but the number of EVs rise just before adipogenesis and are enriched with signaling [...]