/Tag: Trypan Blue

Celigo evaluates a plant extract for glioblastomoa multiforme treatment

At the Canary Center at Stanford for Early Cancer Detection, investigators studied how AshwaMAX (a steroidal lactone from a winter cherry plant, Withania somnifera, extract) might work as an oral treatment for those with the highly aggressive cancer glioblastoma multiforme (GBM). A heterogeneous disease, non-specific therapies for GBM have proven largely ineffective. Two patient-derived GBM lines (GBM2, GBM39) and one GBM cell line were cultured to create neurospheres that were then exposed to various concentrations of AshwaMAX.  Celigo measured cell proliferation and cell death via Trypan Blue staining. AshwaMAX inhibited the neurospheres at nanomolar concentrations. After additional work in vivo, [...]

Cellometer K2 aids in Anti-Cancer Immunity Research

The MD Anderson Cancer Center investigated how a patient’s anti-cancer T cells might be better protected from the cytotoxic effects of the anti-thymidylate drugs (AThys; such as methotrexate) used to treat lung, breast, colon, and pancreas cancers. Although AThys successfully attack cancer cells, the drugs also reduce a patient’s own anti-cancer T cell population, which plays an important role in helping the patient overcome the disease. Using Jurkat, AaPC cells, and mutated human proteins called muteins, researchers were able to manufacture T cells that were resistant to cytotoxic levels of AThys. The Cellometer K2 and Trypan Blue were used to [...]

Researchers in Turkey use Cellometer for Trypan Blue Viability

Here’s another great example of how the Cellometer instruments are used to test viability of cells prior to plating. Standard therapy zoledronic acid (ZA) is typically used for metastatic bone disease in breast cancer patients, but it’s mechanism of action is not clear. Here, Turkish scientists investigated zoledronic acid with and without serine-threonine inhibitors in human MCF-7 and MDA-MB-231 breast cancer cells. Cellometer was used in combination with tryan blue to assess cell viability prior to plating. After plating, the cells were treated with various combinations of therapeutic agents. ZA with either one of the serine-threonine inhibitors examined proved more [...]

By |2015-11-10T10:21:48+00:00November 10th, 2015|Categories: Cellometer User Publications|Tags: , , |0 Comments

French Institute for Health and Medical Research and the University of Lyon use Cellometer Auto T4 for Trypan Blue Viability

Here’s another great example of how researchers across the world are using the Cellometer instruments before performing their downstream assays: Researchers at the French Institute for Health and Medical Research and the University of Lyon investigated the effects of ultrasound on sonoporation (increasing a cell’s permeability in order to transfer molecules or genetic material into the cells). Using adherent HT-29 cells, as well as the Cellometer Auto T4 and trypan blue, researchers evaluated the cells’ viability 24 hours prior to sonication studies. Varying the acoustic intensities and cavitation regulation allowed them to optimize sonoporation efficiency, regardless of ambient temperature. The [...]

By |2015-11-06T10:16:04+00:00November 6th, 2015|Categories: Cellometer User Publications|Tags: , , |0 Comments

UNM College of Pharmacy uses Cellometer to perform TB and AO/PI Viability

Here's a great example of how the Cellometer is being used by researchers in toxicology. The University of New Mexico College of Pharmacy investigated the mechanisms by which environmentally-relevant concentrations of inorganic arsenic contribute to genotoxicity. Inorganic arsenic is present in the air, food, and drinking water in many countries and has been linked to various cancers, skin lesions, and immune system disorders, although the exact mechanisms are not yet clear. To study arsenic’s genotoxic mechanisms, the researchers employed Nexcelom’s Cellometer to assess cell number and viability of primary mouse thymus cells and the murine thymus pre-T cell line, D1 [...]

By |2015-10-19T14:42:41+00:00October 19th, 2015|Categories: Cellometer User Publications|Tags: , , , |0 Comments

Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation Determining cell viability is a vital component in many biological experiments that range from standard cell culture to the investigation of pharmacological agents on tumor cells. One of the earliest and most common methods for measuring cell viability is the trypan blue (TB) exclusion assay [1, 2]. Over the last two decades, there have been various publications on comparing TB exclusion and fluorescence-based cellular viability assays [1, 3-5]. Previous results have shown that in a time-course measurement, TB exclusion [...]