Many factors and considerations must be optimized to develop the appropriate cell-based assays for gene therapy development.
Cellometer Vision CBA was used to count and determine the viability of collected honey bee sperm.
Did your lab’s grant fall through? Funding not an option? We get it – money’s tight right now. In Nexcelom’s long line of automated cell counters, there is one instrument designed specifically with your budget in mind – the Cellometer Mini! As the lowest-cost cell counter on the market, it utilizes bright field imaging, and is perfect for any lab looking to achieve basic cell count, concentration and Trypan Blue viability of cultured cell lines and purified primary samples. It’s affordable, accurate, and small enough to fit in your purse or backpack! Need a little convincing? Don’t just take our word [...]
https://youtu.be/4xUYjVIgOdQ If you're using a microscope and hemacytometer to count your cells and determine viability, you might have felt like Sheldon, when he vacations in Amy's lab and, among other things, helps out by counting the bacteria spores. Everything's going well until Amy returns to the lab, distracts Sheldon, and then he has to start over with his count. Fortunately for you, you don't have to be like Sheldon if you have an automated cell counter. We have bright field counters for cultured cells and Trypan Blue exclusion assays. We have dual fluorescent cell counters for messy, primary samples [...]
Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample.
How safe is your mouthwash? Researchers in Germany worked to answer that question. University of Leipzig (Germany) researchers investigated the cytotoxic effects of different antimicrobial mouth rinses (MRs) on gingival fibroblasts and epithelial cells. Although effective at killing infectious agents, most MRs have some cytotoxic impact on host tissue, which could delay the healing process the rinses are meant to enable. Human primary gingiva fibroblasts and human primary nasal epithelial cells were exposed to various MRs (Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET), and control) for varying lengths of time. The Cellometer Auto T4 analyzed cell [...]
Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements . Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]
Avery Brewing Company in Boulder, Colorado has introduced a Cellometer to their brewing process, improving their procedures, as well as the product’s taste and quality. The Cellometer is employed as a quality control check over the yeast cells inside the facility’s fermenters. Making sure the yeast cells fermenting the beer are healthy and enzymatically active ensures a high quality product, with fewer mutated and/or dying cells that can negatively affect the beer’s flavor. Adam, the owner of Avery Brewing Co. says that the Cellometer has “changed the way we made beer and it also changed the way our beer tastes”. Watch this episode of [...]
Measuring Physiological and Metabolic Characteristics of Yeast Health for Beer Fermentation Samples using the Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Measuring Physiological and Metabolic Characteristics of Yeast Health for Beer Fermentation Samples using the Cellometer Vision Characteristics such as the viability, vitality, glycogen, neutral lipid, and trehalose content of yeast samples must be measured to better understand changes in physiological and metabolic status during fermentation [4-9]. Monitoring changes of these parameters during fermentation can improve current production processes, and a subset of these parameters (glycogen, neutral lipid, and trehalose content) has been shown to play an important role in predicting yeast viability both during and after fermentation [10, 11]. Download our [...]