Using the Cellometer Vision Image Cytometer for Immunophenotyping

It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]

By |2015-10-21T10:30:34+00:00October 21st, 2015|Categories: Cellometer Application News|Tags: , , , , , , |0 Comments

Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »

By |2015-10-07T10:30:08+00:00October 7th, 2015|Categories: Cellometer Application News|Tags: , , , , |0 Comments

Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »

By |2015-09-23T10:30:58+00:00September 23rd, 2015|Categories: Cellometer Application News|Tags: , , , , |0 Comments

Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer In this study, using the CHO cell line, the Celígo Imaging Cytometer was used to monitor transfection efficiency, identify passaging times, optimize media, identify single cells and track clone outgrowth, following previous studies detailing Celígo's performance in tracking cell lineage and confluence [3, 4]. After transfection, cells were allowed to stabilize, then imaged in bright field and fluorescence to monitor and evaluate transfection efficiencies. As wells grew asynchronously, bright field imaging was used to track which wells needed passaging. As [...]

By |2015-09-09T10:30:42+00:00September 9th, 2015|Categories: Celigo Application News|Tags: , |0 Comments

Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements [12]. Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]

By |2015-08-19T10:30:23+00:00August 19th, 2015|Categories: Cellometer Application News|Tags: , , |0 Comments

A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer In this study, Celigo was used to screen a compound library for effects on cell proliferation in adherent and non-adherent cell lines. Human lung carcinoma (A549) and promyelocytic leukemia (HL-60) cells were treated with a panel of compounds to inhibit proliferation. Finally, the Celigo system used image-based analysis to measure changes in cell morphology upon compound treatment. These data indicate that certain anti-proliferative compounds can have secondary effects on cell health or physiology, [...]

By |2015-08-05T10:30:08+00:00August 5th, 2015|Categories: Celigo Application News|Tags: , , , |0 Comments

Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. Previously, we have reported a rapid and accurate fluorescence-based cell population analysis method using a novel image-based cytometry system. The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. Here we report the development of a novel method for the kinetic measurement of cell [...]

By |2018-06-20T20:32:04+00:00July 22nd, 2015|Categories: Cellometer Application News|Tags: , , , , |0 Comments

3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: 3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer U87MG cells were used to create tumorspheres in 384-well plates that were subsequently analyzed by imaging. The data illustrate that reproducible 3D spheroids can be formed in 384-well plates. Fluorescent viability studies were carried out with the imager using pixel intensity analysis. Moreover, the assay was validated for drug screen using various drug compounds that have shown anti-proliferative effects. Together, these data demonstrate that the tumorsphere formation assay can be developed, validated and used for high-throughput anti-cancer compound [...]

By |2018-06-20T20:31:14+00:00July 8th, 2015|Categories: Celigo Application News|Tags: , , , |0 Comments

Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis [1, 2]. Originally, the system has been used for automated cell concentration and viability measurement using disposable counting slides with both bright-field (BR) and fluorescent (FL) imaging methods [3]. The system has recently been demonstrated to perform fast and accurate cell population analysis comparable to the traditional flow cytometry method. Download our white paper »

By |2018-06-20T20:30:09+00:00June 24th, 2015|Categories: Cellometer Application News|Tags: , , |0 Comments

Non-Disruptive Quantification of 2° Reprogrammed iPS Colonies Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Non-Disruptive Quantification of 2° Reprogrammed iPS Colonies Using Celigo Imaging Cytometer Nexcelom's Celigo S imaging cytometer has been applied to provide automated, rapid assessment of iPSC reprogramming. Using f-theta optics, Celigo provides high quality, whole well images using bright field and/or fluorescent illumination. Automated segmentation and analysis provides quantitative output of iPSC reprogramming based on mOrange and GFP fluorescent colony detection. Download our white paper »