Dual-fluorescent CalceinAM/PI Assay for Cell Concentration and Viability
Video Category: Cellometer Assay Principal
A Cellometer assay principal illustration of a dual-fluorescent CalceinAM/PI assay for cell concentration and viability Calcein-AM penetrates the cell membrane of both living and dead cells. Once inside a living cell, Calcein-AM is hydrolyzed by cellular esterases into the cell membrane-impermeable Calcein. PI is only permeable to dead cells.