During this webinar, Dr. Thomas shares his research in cancer biology related to DNA damage in the onset of solid tumors. This research employed a genetic strategy to generate an aneuploidy model and to study the consequences of losing whole chromosomes on tumorigenesis in mammalian cells.
Dr. Thomas also discusses how to perform these ‘day-to-day’ experiments using image cytometry to better streamline your workflow and obtain publication quality images/results while saving a ton of time!
Some of the assays we discuss include:
- DNA damage analysis (counting 𝛾-H2AX foci)
- Apoptosis and other immunofluorescence assays
- 3D/tumor spheroid imaging and counting
- Cell cycle analysis
- Immunophenotyping and other flow cytometry applications
Speaker: Rozario Thomas, PhD
Rozario Thomas is originally from Chennai, South India and obtained his PhD from Weill Cornell Graduate School of Medical Sciences in New York. He performed his thesis research at Memorial Sloan Kettering Cancer Center (MSKCC, New York) on the consequences of an abnormal chromosome number on tumorigenesis using mouse models. For his post-doctoral training he joined the Stanford Cancer Institute to work on understanding the tumorigenic potential of the leukemic stem cells using gene editing technology. He currently works for Nexcelom as a Field Application Scientist for the San Francisco Bay Area and has been leveraging his cancer biology expertise to provide technical support to Nexcelom’s clients.