ViaStain™ Viability Dyes
Nexcelom offers several dyes for fluorescence viability determination.
AO (acridine orange) is a nuclear staining dye used to identify and count nucleated cells in a heterogeneous cell population.
PI (propidium iodide) is a nuclear staining dye that only enters cells with compromised membranes. It is used to identify dead nucleated cells.
AO/PI is a mixture of acridine orange and propidium iodide nuclear staining dyes. The dye mixture can be used with the Cellometer Auto 2000 and Cellometer Vision dual-fluorescence instruments to accurately count live and dead nucleated cells within heterogeneous samples containing non-nucleated cells, such as red blood cells and platelets.