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Platelets

Platelets

Human platelet dilution measurement

Procedure:
  1. Prepare cell sample: fresh PBMC.
  2. Dilute samples with PBS at different dilution factors.
  3. Pipette 20 µL of solution into sample introduction port of a disposable counting chamber.
  4. Insert loaded counting chamber into Auto M10.
  5. Run Auto M10 software to obtain cell concentration results.

A total of 5 two-fold dilutions are used to generate the plot. The result shows excellent linearity for concentration versus sample dilution.


Fresh human platelets imaged by Cellometer® Auto M10, (a) raw image, and (b) after counting. The counted platelets are indicated by green circles.