Nexcelom Bioscience
Surface Marker Cellometer Applications

Image Cytometry for Surface Marker and Intracellular Protein Detection

Compatible with Cellometer:

Introduction

Surface and intracellular proteins are markers that are important for identifying specific type of cells and characterizing cell populations. Traditionally, these assays are performed on flow cytometers. In the recent years, image-based cytometry method has been utilized for many standard surface and intracellular protein detection assay to provide a more simple and rapid method for cell population analysis. The Cellometer Vision CBA image cytometer is specifically designed to perform surface marker and intracellular protein fluorescence analysis to characterize cell populations. Below are examples of some of the assays have been performed using the Cellometer image cytometry method.

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Immunophenotyping for Immunological Research

Biomarkers related to surface protein express on various types of immune cells such as T Cell, Activated T Cell, B Cell, Lymphocytes, splenocytes, thymocytes, etc. can be detected and measured on the Cellometer Vision CBA image cytometer. The data shown below are some examples of fluorescent intensity data measured from bright-field and fluorescent images captured by the Cellometer Vision CBA image cytometry method. These biomarkers are important to characterize cell samples for downstream assays such as immune cell purification, activated immune cell populations, and cell sorting.

Human Activated T Cell – CD44-APC

human activated T cells - CD44-APC

Bright Field Counted Image

Bright field counted cell image of human activated T cells

Fluorescence Image

Fluorescence cell image of human activated T cells

Human Activated T Cell – CD44-PE

Human Activated T Cell – CD44-PE

Bright Field Counted Image

Bright field counted cell image of Human Activated T Cell – CD44-PE

Fluorescence Image

Fluorescence cell image of Human Activated T Cell – CD44-PE

Macrophage in Mouse Bone Marrow – CD11b-PE

Macrophage in Mouse Bone Marrow – CD11b-PE

Bright Field Counted Image

Bright field counted cell image of Macrophage in Mouse Bone Marrow – CD11b-PE

Fluorescence Image

Fluorescence cell image of Macrophage in Mouse Bone Marrow – CD11b-PE

Human Jurkat Cells – CD3-PE

Human Jurkat Cells – CD3-PE

Bright Field Counted Image

Bright field counted cell image of Human Jurkat Cells – CD3-PE

Fluorescence Image

Fluorescence cell image of Human Jurkat Cells – CD3-PE

Human PBMC – CD3-FITC

Human PBMC – CD3-FITC

Bright Field Counted Image

Bright field counted cell image of Human PBMC – CD3-FITC

Fluorescence Image

Fluorescence cell image of Human PBMC – CD3-FITC

Human PBMCs – CD3-PECy5

Human PBMCs – CD3-PECy5

Bright Field Counted Image

Bright field counted cell image of Human PBMCs – CD3-PECy5

Fluorescence Image

Fluorescence cell image of Human PBMCs – CD3-PECy5

Human PBMCs – CD19-PECy5

Human PBMCs – CD19-PECy5

Bright Field Counted Image

Bright field counted cell image of Human PBMCs – CD19-PECy5

Fluorescence Image

Fluorescence cell image of Human PBMCs – CD19-PECy5

Mouse Cells – Mouse IgG-FITC and RFP

Mouse Cells – Mouse IgG-FITC and RFP scatter plot

Bright Field Counted Image

Bright field counted cell image of Mouse IgG-FITC and RFP

Fluorescence Image

Fluorescence cell image of Mouse IgG-FITC and RFP

Fluorescence Image

Fluorescence cell image of Mouse IgG-FITC and RFP

CD44 - PE

CD44 - PE

Bright Field Counted Image

Bright field counted cell image of CD44 - PE

Fluorescence Image

Fluorescence cell image of CD44 - PE

Human Lymphocytes – CD4-PE

Human Lymphocytes – CD4-PE

Mouse Thymocytes – CD8-PE and CD4-APC

Mouse Thymocytes – CD8-PE and CD4-APC

Mouse Splenocytes – CD8-PE and CD4-APC

Mouse Splenocytes – CD8-PE and CD4-APC

MHC Dextramer – CD8-FITC and Dextramer-PE

MHC Dextramer – CD8-FITC
MHC Dextramer – CD8-FITC
MHC Dextramer – Dextramer-PE

Human T Cells and Cytokines - CD8-PE and INFg-FITC

Human T Cells and Cytokines -  CD8-PE and INFg-FITC

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Intracellular Protein for Protein Pathway Research

Various complex proteins exist in the cells that are generated or removed by cellular functions. These proteins can be an indicator to measure certain protein pathways or functions of the cells to determine if they are operating normally or abnormally. These intracellular proteins can be fluorescently labeled and measured by Cellometer Vision CBA image cytometer to determine the cell population associated with these proteins.

Heat Shock Protein in MNNG Cancer Cells – HSP90-PE and GFP

Heat Shock Protein in MNNG Cancer Cells – HSP90-PE and GFP

Bright Field Counted Image

Bright field counted cell image of Heat Shock Protein in MNNG Cancer Cells

Fluorescence Image

Fluorescence cell image of Heat Shock Protein in MNNG Cancer Cells

Fluorescence Image

Fluorescence cell image of Heat Shock Protein in MNNG Cancer Cells

Heat Shock Protein in MNNG Cancer Cells – Grp78-Alexa Fluor 594 and GFP

Heat Shock Protein in MNNG Cancer Cells – Grp78-Alexa Fluor 594 and GFP

Bright Field Counted Image

Bright field counted cell image of Heat Shock Protein in MNNG Cancer Cells

Fluorescence Image

Fluorescence cell image of Heat Shock Protein in MNNG Cancer Cells

Fluorescence Image

Fluorescence cell image of Heat Shock Protein in MNNG Cancer Cells

Jurkat Cells Early Apoptosis Marker – Caspase 3-FITC

Jurkat Cells Early Apoptosis Marker – Caspase 3-FITC

Bright Field Counted Image

Bright field counted cell image of Jurkat Cells Early Apoptosis Marker – Caspase 3-FITC

Fluorescence Image

Fluorescence cell image of Jurkat Cells Early Apoptosis Marker – Caspase 3-FITC

Jurkat Cells Early Apoptosis Marker – Caspase 8-FITC

Jurkat Cells Early Apoptosis Marker – Caspase 8-FITC

Bright Field Counted Image

Bright field counted cell image of Jurkat Cells Early Apoptosis Marker – Caspase 8-FITC

Fluorescence Image

Fluorescence cell image of Jurkat Cells Early Apoptosis Marker – Caspase 8-FITC

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General Surface Marker

Jurkat Cells Late Apoptosis by Phosphatidylserine – Annexin V-FITC and Propidium Iodide

Jurkat Cells Late Apoptosis by Phosphatidylserine

Bright Field Counted Image

Bright field counted cell image of Jurkat Cells Late Apoptosis by Phosphatidylserine

Fluorescence Image

Fluorescence cell image of Jurkat Cells Late Apoptosis by Phosphatidylserine

Fluorescence Image

Fluorescence cell image of Jurkat Cells Late Apoptosis by Phosphatidylserine

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Stem Cell Analysis for Regenerative Medicine

Stem cell markers are often used to determine the stem cell population in blood, cord blood, bone marrow, and adipose tissue. These cells are extremely important for research in regenerative medicine, and the ability to quickly characterize a stem cell population in a cell sample can greatly improve research efficiency. Cellometer Vision CBA image cytometer can be utilized to measure cell population of some of the common stem cell markers.

Mouse iPS Cells – SSEA-1-PE

Mouse iPS Cells – SSEA-1-PE

Bright Field Counted Image

Bright field counted cell image of Mouse iPS Cells – SSEA-1-PE

Fluorescence Image

Fluorescence cell image of Mouse iPS Cells – SSEA-1-PE

Human iPS Cells – SSEA-4-PE

Human iPS Cells – SSEA-4-PE

Bright Field Counted Image

Bright field counted cell image of Human iPS Cells – SSEA-4-PE

Fluorescence Image

Fluorescence cell image of Human iPS Cells – SSEA-4-PE

Human iPS Cells – TRA-181-PE

Human iPS Cells – TRA-181-PE

Bright Field Counted Image

Bright field counted cell image of Human iPS Cells – TRA-181-PE

Fluorescence Image

Fluorescence cell image of Human iPS Cells – TRA-181-PE

CACO2 Cancer Stem Cells – CD133-FITC

CACO2 Cancer Stem Cells – CD133-FITC

Bright Field Counted Image

Bright field counted cell image of CACO2 Cancer Stem Cells – CD133-FITC

Fluorescence Image

Fluorescence cell image of CACO2 Cancer Stem Cells – CD133-FITC

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