Accurately Measure Cell Size of NCI-60 Cancer Cell Lines

In this experiment average cell diameter for A498 cells was measured to be 19.9 microns. As we can see from the Cell Diameter Histogram approximately 70% of the cells had a cell diameter that was between 17.5 and 21.3 microns.


NCI-60 A498 cell size histogram

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Trypan Blue stained A498 cells   Cellometer counted A498 cells

Trypan Blue stained A498 cells Cellometer counted A498 cells

Furthermore, since cell size parameters can be customized in the Cellometer software, the user has the capability to count only those cells that fall within the designated cell size. In this case (shown below) only cells that are between 15 and 30 microns were counted.

Counted A498 Cells > 15 µm in Diameter

A498 only cells less than 15 µm counted

Counted A498 cells are delineated by green outlines. Cells that are less than 15 µm were not counted.