Transwell Migration2018-06-10T22:43:58+00:00

Transwell Migration

Image and Quantify Transwell Invasion and Migration Assays using Suspension and Adherent Cells

  1. Image transwell invasion of adherent cells without dissociation of cells from the transwell insert
  2. Directly count the number of invading/migrating cells
  3. Fluorescently label and image invading cells
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Celigo Product Guide
Applications Collection

Perform and Quantify Transwell Invasion Assay by Direct Cell Count

Transwell Invasion Assay 2

Suspension cells located inside the insert migrate through the porous membrane toward the chemoattractant from the bottom plate. Celigo is used to automatically count migrated cells in the bottom plate.

Image and Count the Number of Cells in a Transwell Invasion Assay

Bright field and DAPI images of transwell invasion of adherent cancer cells

Bright field and DAPI images of transwell invasion

Overlay of bright field and DAPI

Bright field and DAPI overlay image of transwell invasion

High-resolution bright field images of the transwell membrane

High resolution bright field images of the transwell membrane

Thumb-nail pictures and cell numbers are automatically displayed upon the completion of the cell counts

Acquisition of high-resolution DAPI and bright field images of the transwell membrane surface in a 24 well plate format took ~ 3 minutes