Perform and Quantify Transwell Invasion Assay by Direct Cell Count
Suspension cells located inside the insert migrate through the porous membrane toward the chemoattractant from the bottom plate. Celigo is used to automatically count migrated cells in the bottom plate.
Image and Count the Number of Cells in a Transwell Invasion Assay
Bright field and DAPI images of transwell invasion of adherent cancer cells
Overlay of bright field and DAPI
High-resolution bright field images of the transwell membrane
Thumb-nail pictures and cell numbers are automatically displayed upon the completion of the cell counts
Acquisition of high-resolution DAPI and bright field images of the transwell membrane surface in a 24 well plate format took ~ 3 minutes