Fluorescent staining can be used to simplify traditional methods by mixing a cell membrane permeable DNA dye (acridine orange and propidium iodide) with diluted whole blood sample that specifically stains white blood cells. This is an ideal solution for many complex cell population characterization assays such as rapidly counting white blood cells in whole blood. Nexcelom’s Cellometer automated cell counters are able to quantitatively analyze WBCs by simply pipetting 20 µl of treated sample into a Cellometer disposable counting chamber and placing the chamber into the instrument. Since the counting chamber is disposable, no washing is required between samples, there is no risk of cross contamination of different samples, and the risk of exposure to biohazard materials is reduced.
Using proprietary algorithms, the Cellometer’s robust operating software accurately analyzes cell images, and generates counting data in less than 60 seconds typically. Cell images and all analysis data, including cell size distribution histograms, can be saved for documentation. Data can also be easily exported to Microsoft Excel spreadsheets for further analysis.