The Cellometer line of automated cell counters incorporates image based cell counting and fluorescence detection in a compact and easy-to-use instrument that reliably automates cell counting, viability determination and other fluorescent assays. By simply pipetting 20µL of sample into a disposable chamber, Cellometer acquires cell images that are analyzed to determine cell counts, concentration, cell sizes, and fluorescence intensity, typically in 30 seconds or less, making them an ideal instrument for low volume samples. Similarly, 20 µL is needed to perform GFP trasduction/transfection efficiencies, cell cycle analysis or any other cell-based-assay.
This combination of features makes it ideal for use in stem cell research. Samples can be counted and viability determined with Trypan Blue using the bright field Cellometer cell counters. Our fluorescent Cellometer cell counters and image cytometer systems combine this bright field functionality with fluorescent detection to also determine stem cell viability using fluorescent stains such as Propidium Iodide, and being able to accurately determine concentration of nucleated cells in human bone marrow or cord blood. The fluorescence imaging mode can also be used to quantify GFP transfection efficiency when transfecting stem cells.