Primary stromal vascular fractions (SVFs) are characterized by the complexity of cell types that are typically found within each sample. This complexity does not come without some drawbacks. As seen in the above bright field images, SVF samples have a lot of cellular debris which significantly complicates the ability to acquire the correct cell count and viability.
In this study we compared the Cellometer Vision CBA platform against manual counting using hemacytometer as well as a flow cytometer. The results show that there is a great correlation between these counting methods with the greatest similarity seen between the Cellometer platform and the flow cytometer.
By using cell nucleus identifying dyes like AO/PI and Hoechst/PI it significantly increases the confidence of proper and accurate counting and viability. Additionally, the ability to gate out small debris in both the bright field and fluorescent channels assures that the correct cell count is obtained.
The ability to perform rapid staining (AO/PI staining is instantaneous) and analysis (<30 seconds for imaging and analysis) while using a small sample volume (20 µL) makes the Cellometer Vision CBA an ideal system to obtain cell concentrations and viability of stromal vascular fractions.