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Cell Counting Method: Fit for Purpose
Cell Counting Method: Fit for Purpose
Polly Mitchell
2021-02-02T17:01:12+00:00
Cell Counting Method Assessment Worksheet
The selection of cell counting methods should be fit-for-purpose, and the first step is to understand or clarify the intended purpose of the measurements. Please fill out the “Cell Counting Needs Assessment Worksheet” and submit the form. Our field application scientists will reach out and work with your needs to determine the best cell counting method and strategies for your work.
1. Projects conducted in your lab
Research
Analytical method development-CMC
Process development (PD)
Clinical trial site
2. Standardize cell counting assays for
Incorporating best practices for cell counting measurement quality
Multiple labs in the same or several locations doing the same bioassays
The ability to transfer assays from discovery to development, clinical trial sites and manufacturing
Assay improvements to be leveraged by the entire organization
3. Intended cell counting results
Live cell count/concentration
Dead cell count/concentration
Total cell count/concentration
% Viability of entire or sub-population
Cell count/concentration of biomarker/protein expression
% Biomarker/protein expression
4. Select the applications for your lab
(How will you use the cell counting results?)
Cell culture of cell lines and purified primary cells
Whole tissues with nucleated cells
Unfractionated whole blood
- Bone marrow aspirate
- Cord blood
- Adipose tissue
- Hepatocytes
Isolation, cryopreservation and thawed human PBMCs and platelets
- From whole blood
- From Ficoll or density-gradient separation process
- From leukapheresis
Mouse tissue processing into single cell suspension
- Bone marrow
- Spleen
- Lymph nodes
- Whole blood
- PBMCs
- Other tissue digests
Tumor digestion into single cell suspension
- Nucleated cells in brochoalveolar lavage (BAL)
Immunophenotyping using a flow cytometer / CyTOF
- PBMCs
- Unfractionated whole blood
- Sort cells using FACS
Single-cell analysis
- Transcriptome analysis using droplet technology Single-based sequencing
Plating cells for
- ELISPOT assay
- Cytotoxicity assays
Normalization of cell numbers for
- Protein/ DNA/ Cellular respiration/metabolism based assay readout
- Whole blood stimulation of inflammatory cytokine production
Cellular therapy manufacturing process
- Human bone marrow stromal cells
- Human NK, CAR-NK and CAR T cell therapies
- Genetic engineered TCR expressing T cells
- Tumor-infiltrating lymphocytes (TIL-T cells)
- CAR T cells derived from iPSC
5. Other assays not listed above:
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