Poster: Long term time-course monitoring of NK cell-mediated ADCC using the Celigo Image Cytometer

Antibody-dependent cell-mediated cytotoxicity (ADCC) assay has been widely performed for immuno-oncological research. Traditionally, ADCC assays are conducted by measuring the amount of released Chromium, calcein AM, or Lactate dehydrogenase (LDH) molecules 4 hours after the target cancer cells are killed by effector/antibody pair. Not only are these methods inaccurate due to indirect measurement of supernatant, but there is a need to characterize the effects of antibody-dependent cytotoxicity at much longer time periods (≥ 72 hours).