We have identified that inherent RBC contamination in fresh human PBMC can vary from 0 to greater than 30%.
Results of 15 human PBMC samples were analyzed for their RBC contamination %
4 samples showed High contamination (> 30%)
6 samples showed Medium contamination (10 – 30%)
5 samples showed Low contamination (< 10%)
(Journal of Immunological Methods, Volume 388, Issues 1–2, 28 February 2013, Pages 25-32)
Acridine Orange/Propidium Iodide Dual Fluorescent stain provides an assay method to identify live and dead nucleated cells without interference from red blood cells.
Cellometer cell counters employ AO/PI method to automatically count PBMC & Measure Viability without Lysing RBC.