Nexcelom Bioscience
Hemacytometer and other cell counting methods

How do I Count Cells with a Manual Hemacytometer?

Manual Cell Counting with Manual Hemacytometer

Order your calculation notepad

Step 1. Prepare the Hemacytometer

Clean hemacytometer and glass cover slip with 70% EtOH.

Step 2. Prepare Sample & Load Hemacytometer

Pipette 10 microliters of sample into the manual hemacytometer.

Step 3. Manually Count Cells in Sample

a. Place the hemacytometer under a microscope with a typical magnification of 100.
b. Focus both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares

Step 4. Cell Calculations & Disposal of Hemacytometer

a. Multiply the dilution factor by the total number of cells, divide by the # of corner squares counted, and multiply by 10E4 to obtain cell concentation (cells/ml).
b. Clean hemacytometer and glass cover slip with 70% EtOH.

How do I Count Cells with a Cellometer® Disposable Hemacytometer?

What Makes Our Hemacytometer Different?

  • All-in-one design
  • No cleaning or assembly of parts
  • Two counting chambers per slide
  • No washing biohazard
  • Easily disposed of after use

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Manual Cell Counting with a Cellometer Disposable Hemacytometer

Step 1. Prepare Sample & Load Hemacytometer

Pipette 20 microliters of sample into the Cellometer disposable hemacytometer

Step 2. Manually Count Cells in Sample

a. Place the disposable hemacytometer under a microscope with a typical magnification of 100.
b. Focused both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares

Step 3. Cell Calculations & Disposal of Hemacytometer

a. Multiply the dilution factor by the total number of cells and divide by the sample volume.
b. Dispose the hemacytometer.

Details of the Cellometer Disposable Hemacytometer

Disposable Hemacytometer with two cell counting chambers per slide for replicates Nexcelom Bioscience's Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.

Cell counting grid pattern is the Improved Neubauer, as in a common Hemacytometer
The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.

Cellometer® CP2 Disposable Hemacytometer

Catalog #
Description
Unit
Cellometer CP2-001
Plastic cell counting chamber slides with 2 chambers per slide containing a hemacytometer grid on the slide
Box of 25 slides for 50 counts
Cellometer CP2-002
Plastic cell counting chamber slides with 2 chambers per slide containing a hemacytometer grid on the slide
Box of 100 slides for 200 counts

Ready to Automate Your Cell Counting?

Automated Cell Counting using Cellometer Automated Cell Counters

  • Improve accuracy & increase throughput
  • Automatically calculate concentration & viability
  • Save images & cell size histograms
  • GFP transfection rates & fluorescence cell counts

Pipette 20 µL
Insert Slide
Get Data