Importance of Cell Cycle Research
Since cancer cells often undergo abnormal cell division and proliferation, it is important to understand their cell cycle.
Studies involving characterization of pharmacological reagents and their effect on cell cycle regulation.
Arrest normal cell cycling during chemotherapy treatment.
To understand cellular, molecular, and biological mechanisms, it is essential to examine their role in cell cycle.
Current Detection Methods
3H-thymidine – Radioactive labeling of thymidine. Upon DNA replication, the radiolabled thymidine is incorporated into new DNA.
Live cell measurement
Hoechst 33342 – Is cell permeable and therefore do not require cell fixation. Binds to the minor groove of double-stranded DNA.
Fixed cell measurement
Bromodeoxyuridine (BrdU)– A thymidine analog, is incorporated into the genome during the S-phase of the cell cycle. Detected using anti-BrdU antibodies.
DAPI – Binds to the AT rich regions of the DNA. Poor live cell permeability and therefore often used post-fixing.
Propidium Iodide (PI) – A membrane exclusion dye and an intercalating agent that stains the cellular genome upon cell fixation.
Fluorescence-Based, Fixed Cell Measurement Using Cellometer