//Cellometer Fluorescent Viability Counters
Cellometer Fluorescent Viability Counters2018-07-06T15:18:42+00:00

Cellometer Fluorescent Viability Cell Counters

Nexcelom’s fluorescent viability cell counters take our bright field imaging systems to the next level with fixed single or dual fluorescent optics modules. This allows researchers to use fluorescent stains to determine viability easily, without the need to perform RBC lysis. The Cellometer Auto 2000 automated cell counter provides an all-in-one touch-screen solution for PBMC counting while the Cellometer K2 is optimized for complex primary samples such as hepatocytes. The Cellometer X1 and X2 automated cell counters have higher magnification for use with small cells such as yeast and platelets.

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Viability of primary cells containing debris and red blood cells.

all-in-one viability cell counter

Optimized for brewing yeast, wine yeast, platelets and other small cells.

automated fluorescent viability cell counter

Analysis of hepatocytes, stem cells, splenocytes, tumor suspension and other complex primary cells.

fluorescent viability cell counter

Cellometer Fluorescent Viability Cell Counters at a Glance

Cell / Sample Type Auto 2000 X1 X2 K2
Cell Line
X
Cultured Primary Cells
X
Platelets
X
 
Low Concentration Cell Lines
X
 
X
Yeast (Clean Sample)
X
X
 
Yeast (Messy Sample)
X
Primary cells (Messy Sample*)
X
X
PBMCs, Splenocytes, Stem Cells
X
 X
Hepatocytes
X
Adipocytes***
X
X
Cell-Based Assay **
X
X
X
Apoptosis (Annexin V-FITC/PI)
X
Apoptosis (Caspase Activity)
X
Cell Cycle (PI)
X
X
X
GFP Transfection
X
X
X
Vitality (Calcein-AM/PI)
X
X
Vitality (CFDA-AM)
X
Imaging Capabilities
 Auto 2000  X1  X2
K2
Fluorescence Intensity Measurements
X
X
X
Configuration
 Auto 2000  X1  X2
K2
Objective Magnification
4X
10X
10X
4X
Bright field
X
X
X
X
Contains Single Fluorescence Optics Module
X
Contains Dual Fluorescence Optics Module
X
X
X
Integrated Computer / Touchscreen
X
Optional Laptop Computer
X
X
X
Software Features
 Auto 2000  X1  X2
K2
Cellometer software for analysis of clumpy and irregular-shaped cells
X
X
X
X
Mean cell diameter and cell size distribution
X
X
X
X
Sample adjustment calculator to determine sample volume for desired cell number/concentration
X
X
X
X
Cell type wizard for creating new cell type parameters
X
X
X
IQ / OQ Option
X
X
X
X
GLP/GMP Audit Control Option
X
X
X
Specialized algorithms for analysis of adipocytes***
X
X
Specialized algorithms for analysis of hepatocytes
X
FCS Express Software for flow-like data reporting for cell-based assays
X
X
X
Performance Criteria
 Auto 2000  X1  X2
 K2
Analysis time
5-15 sec.
5-15 sec.
5-15 sec.
5-15 sec.
Cell size range
5-300 µm
0.5-15 µm
0.5-15 µm
5-300 µm
Sample volume
20 µl
20 µl
20 µl
20 µl
*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell-Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter