Cellometer Fluorescent Viability Cell Counters

Nexcelom’s fluorescent viability cell counters take our bright field imaging systems to the next level with fixed single or dual fluorescent optics modules. This allows researchers to use fluorescent stains to determine viability easily, without the need to perform RBC lysis. The Cellometer Auto 2000 automated cell counter provides an all-in-one touch-screen solution for PBMC counting while the Cellometer K2 is optimized for complex primary samples such as hepatocytes. The Cellometer X1 and X2 automated cell counters have higher magnification for use with small cells such as yeast and platelets.

I like the cell images with AO/PI dye. When a cell line didn’t have GFP protein just looking at the images I could tell that there was something wrong with it. I have compared it [Cellometer Auto 2000] with a hemacytometer and they are comparable while Vi-Cell was not.

Elstar Therapeutics

The Auto 2000 is extremely useful for our laboratory. We use one a lot of times during our day. Cellometer helps do experiments much faster than before.

Brigham and Women's Department of Pathology

The X2 has allowed us to increase throughput in our QC lab while maintaining a high degree of accuracy, eliminating much of the inter-operator variability associated with manual cell counting. The time savings and repeatability are tremendous, and the increased confidence in our cell counts in priceless!

Alementary Brewing Co

I love my Nexcelom X2 Cellometer! It allows me to quickly measure yeast viability in my lab and for my customers! No more tedious counting yeast cells under the microscope via methylene blue.

Sleight Beer Lab

Cellometer K2 is very compact, reliable and easy to use. Our lab uses this counter to count live and dead cells for our experiments. It is a very good alternative to flow cytometer for cell cycle and apoptosis studies.

Stanford University
[Cellometer] K2 allows us for a faster, reliable and more accurate cell counts that our lab uses so frequently. The process itself is simple and intuitive compared to the other machine we used to have. Such a great improvement!

Northwestern University
Request a Virtual or In-lab Demo

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

fluorescent viability cell counter

Analysis of hepatocytes, stem cells, splenocytes, tumor suspension and other complex primary cells.

fluorescent viability cell counter

Viability of primary cells containing debris and red blood cells.

all-in-one viability cell counter

Optimized for brewing yeast, wine yeast, platelets and other small cells.

automated fluorescent viability cell counter

Cellometer Fluorescent Viability Cell Counters at a Glance

Cell / Sample Type

Auto 2000

X1

X2

K2

Spectrum

Cell Line
X
X
Cultured Primary Cells
X
X
Platelets
X
 
X
Low Concentration Cell Lines
X
 
X
X
Yeast (Clean Sample)
X
X
 
Yeast (Messy Sample)
X
Primary cells (Messy Sample*)
X
X
X
PBMCs, Splenocytes, Stem Cells
X
 X
X
Hepatocytes
X
X
Adipocytes***
X
X
X
Cell-Based Assay **
X
X
X
X
Apoptosis (Annexin V-FITC/PI)
X
X
Apoptosis (Caspase Activity)
X
X
Cell Cycle (PI)
X
X
X
X
GFP Transfection
X
X
X
X
Vitality (Calcein-AM/PI)
X
X
X
Vitality (CFDA-AM)
X
X

Imaging Capabilities

 Auto 2000

 X1

 X2

K2

Spectrum

Fluorescence Intensity Measurements
X
X
X
X

Configuration

Auto 2000

 X1

 X2

K2

Spectrum

Objective Magnification
4X
10X
10X
4X
5X
Bright field
X
X
X
X
X
Contains Single Fluorescence Optics Module
X
Contains Dual Fluorescence Optics Module
X
X
X
X
Integrated Computer / Touchscreen
X
Optional Laptop Computer
X
X
X
Laptop Included
X

Software Features

Auto 2000

 X1

 X2

K2

Spectrum

Cellometer software for analysis of clumpy and irregular-shaped cells
X
X
X
X
X
Mean cell diameter and cell size distribution
X
X
X
X
X
Sample adjustment calculator to determine sample volume for desired cell number/concentration
X
X
X
X
X
Cell type wizard for creating new cell type parameters
X
X
X
X
IQ / OQ Option
X
X
X
X
X
GLP/GMP Audit Control Option
X
X
X
X
Specialized algorithms for analysis of adipocytes***
X
X
X
Specialized algorithms for analysis of hepatocytes
X
X
FCS Express Software for flow-like data reporting for cell-based assays
X
X
X
X

Performance Criteria

Auto 2000

 X1

 X2

 K2

Spectrum

Analysis time
5-15 sec.
5-15 sec.
5-15 sec.
5-15 sec.
5-15 sec.
Cell size range
5-300 µm
0.5-15 µm
0.5-15 µm
5-300 µm
5-300 µm
Sample volume
20 µl
20 µl
20 µl
20 µl
20 µl
*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell-Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter