Cellometer Spectrum Image Cytometry System

Customizable Cell Counting and Image Cytometry Instrument


See how the Spectrum Image Cytometer works »
How to setup the Spectrum

How to setup the Spectrum

How to change fluorescent filters

How to change fluorescent filters

Request a Virtual or In-lab Demo
Request a Quote
Modern Cell-Based Assays Virtual Symposium

Features of the Spectrum Image Cytometry System

The Spectrum Image Cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned templates. Quickly plot cell population data as a histogram, scatter plot, dot plot or contour plot using FCS express.

The filter sets are user-changable, allowing you to customize the instrument for specific assays/reagents of interest.

All-in-One System

Basic cell counting, primary cell viability, and cell-based assays.

Dual-Fluorescence for Accurate Primary Cell Viability

No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.

User Changeable Fluorescence Filters

Choose from six color options to run two-color assays

Unique Algorithms for Advanced Cell Analysis

Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.

Fast Results

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

“…the Cellometer Spectrum is an economical flow cytometry alternative for small scale simple cell phenotyping and cell counting.”

Emmett E. Hedblom, Ph.D, TScan Therapeutics

Simple Cell-Based Assays

  • Small 20 µl sample size
  • Cell count, concentration and viability
  • Two-color antibody assays
  • GFP/RFP Transfection
  • Cell health and cell-based assays, including:
    • Apoptosis
    • Cell Proliferation
    • Cell Cycle
    • Mitochondrial Potential
    • Phagocytosis
    • Surface Marker Analysis

Advantages of Image Cytometry

View Cell Images

  • Visually check cell morphology
  • Ensure only cells of interest are counted
  • Archive and re-analyze cell images
  • Export images for publication

Proprietary Pattern-Recognition Software

  • Count individual cells in clusters
  • Count irregular-shaped cells
  • Count cells based on size
  • Eliminate debris from cell counts

Non-Fluidic Platform

  • Disposable counting chambers – no washing
  • Compatible with fragile cells
  • Maintenance-free
  • Robust optics modules and LED light sources

IQ/OQ Validation and GMP/GLP Accessories

  • Installation Qualification reagents/protocol
  • Operational Qualification reagents/protocol
  • On-site IQ or OQ Performance
  • GMP/GLP Software Module

Accurate Cell Counting

Bright Field

Bright field cell images

Stained AO+ Cells

Stained AO positive cells

Counted AO+ Cells

Counted AO positive cells
Broad Dynamic Range
  • Automatic identification of Acridine Orange (AO) stained cells (shown above)
  • Image and count up to 2×107 cells/mL
  • Image acquisition and analysis:
    30 seconds/sample for a 3-channel assay
  • Versatile platform – use for routine cell counting and complex cell-based assays

Proven Results

  • Determine cell viability, for cell lines or primary samples, using AO/PI in seconds
  • Accurately measure cell samples with varying viability (0 – 100%)
Measured Jurkat Cell Viability

Bright Field

Bright Field Jurkat Cells

Stained AO+ Cells

Stained PI Cells

Combined Counted Cells

Combined Counted Cells

Export to FCS Express* for Flow-Like Data

Counted PI+ Cells

Contour Population Plot

Spectrum contour population plot
  • Export image data into flow cytometry software: FCS express
  • Simple workflow: No fluid- stream, No PMT voltages, No forward/side scatter
  • Easily perform data analysis using pre-designed templates
  • Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot

Propidium Iodide (PI) Cell Cycle

PI Cell Cycle Histogram
* FCS Express Flow Cytometry software is a product of De Novo Software and is included with Cellometer Spectrum
Cell Population % of Gated Cells Concentration
(10^6 cells/mL)
Total

Sub G1

G0/G1

S

G2/M

100

3.8

61.9

15.3

19

3.18

0.12

1.97

0.49

0.60

Histogram of PE CD3+ Jurkat cells

Histogram of PE CD3+ Jurkat cells

CD3 Bright Field

Histogram of PE CD3+ Jurkat cells

CD3 PE

CD3 PE
Cellometer Spectrum CD4 and CD8 cell images
% Total
Q1: CD8 + (PE) 34.04
Q3: CD4/CD8 – 20.51
Q4: CD4 + (Green) 37.42

A bright field image (A) of human PBMCs as well as those stained with human anti-CD4-BB515 (B) and anti-CD8-PE antibodies (C) were imaged on the Spectrum. Population analysis was performed using FCS ExpressTM to determine the percentage of CD4 and CD8 positive cells as well as the percentage of cells that were double-negative (D).

Cellometer Spectrum CD4 CD8 scatterplot data
GFP/RFP Population Analysis

Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.

Bright Field

Bright Field Cell Image

GFP/RFP Positive Cells

GFP/RFP Positive Cells

User-Changeable Fluorescence Optics Modules*

The Spectrum is designed to hold two user-changeable fluorescence optics modules. Purchase only the modules you need and easily configure the instrument by quickly changing the colors to fit your experimental design.

Optics Module Fluorophores Nucleic Acid Stains

Fluorescent Proteins and
other Fluorescent Cell Reagents

S1-452-365

Ex: 370 nm (BW: 36 nm)

Em: 452 nm (BW: 45 nm)

BV421

V450

Pacific Blue

Hoechst 33342

DAPI

ViaStain™ Dead Cell Nuclear Blue

Calcein AM Violet

CTV (CellTrace Violet)

Tracer Blue

BFP

S1-534-470

Ex: 475 nm (BW: 42 nm)

Em: 534 nm (BW: 42 nm)

FITC

AlexaFluor® 488

AO (acridine orange)

SYTO®9, SYTO®13

SYTOX®Green

SYTO®BC

GFP

YFP

Calcein AM

CFSE

JC-1

S1-594-470

Ex: 475 nm (BW: 42 nm)

Em: 594 nm (LP – Long Pass)

Chlorophyll A

Chlorophyll B

S1-605-527

Ex: 525 nm (BW: 45 nm)

Em: 605 nm (BW: 64 nm)

AlexaFluor® 546

AlexaFluor® 555

Cy3®

PE (R-phycoerythrin)

RFP

PI (propidium iodide)

EB (ethidium bromide)

SYTOX® Orange

RFP

mCherry

TdTomato

TurboRed

TMRE/TMRM

JC-1

S1-655-527

Ex: 525 nm (BW: 45 nm)

Em: 655 nm (BW: 40 nm)

PI (propidium iodide)

EB (ethidium bromide)

7-AAD

Nile Red

S1-692-620

Ex: 628 nm (BW: 40 nm)

Em: 692 nm (BW: 40 nm)

AlexaFluor® 647

APC (allophycocyanin)

Cy5®

SYTOX® Red

iRFP670

CellTrace Far Red

Cell Tracker Deep Red

LysoTracker Deep Red

*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.
Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.

Cell Types for Many Research Areas

Optimized for primary cell analysis

PBMCs

Stem Cells

Adipocytes

Neural Cells

Hepatocytes

Dendritic Cells

Epithelial Cells

Keratinocytes

Lymphocytes

Splenocytes

Monocytes

Cellometer Spectrum Reagents

Cell Counting / Viability Reagents

Yeast Viability & Vitality Reagents (for 10x instrument)

Apoptosis Reagents

Cell Cycle Reagents

Proliferation/Tracer Reagents

Cell Health/Fitness Reagents

Cellometer Spectrum Specifications

Includes
  • Cellometer Spectrum Instrument
  • Cellometer Software
  • FCS Express 6 analysis software (2 licenses)
  • Two Fluorescent Optics Modules
  • Power Supply
  • USB 2.0 Connection Cable
  • Phone/online applications support during set-up
Magnification Selection
  • 5x (ex: most mammalian cells)
  • 10x (ex: yeast, platelets, algae)
Imaging Performance 5x magnification
Cell Size: 5 – 300* microns, Conc. Range: 105 – 107 cells/ml10x magnification
Cell Size: 0.5 – 25 microns,  Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.* Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter
Instrument Specifications Weight: 24.0 lbs. (10.9 kg)
Dimensions: Width: 6.0” (15.2 cm), Depth: 8.5” (21.6 cm), Height: 14.0” (35.6 cm)
Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
Output to Instrument: 12 VDC, 3.34A
PC / Laptop
Minimum
Requirements(If purchasing Cellometer  without PC laptop)
  • Windows 10
  • 2 GHz or higher processor
  • 4GB RAM (8GB RAM or higher recommended)
  • USB 2.0 or higher
  • Display resolution: 1024 x 768 pixels or higher

Intel Mac with Parallels/Running Windows 10

Customer Publications using Cellometer Spectrum