Cellometer Spectrum
Image Cytometry System for 20 µl Cell-Based Assays
Cell Cycle
Apoptosis
Cell-Mediated Cytotoxicity
Cell Counting and Viability
Interchangeable Fluorescent Modules
Features of the Spectrum Image Cytometry System
All-in-One System
Basic cell counting, primary cell viability, and cell-based assays.
Dual-Fluorescence for Accurate Primary Cell Viability
No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.
Unique Algorithms for Advanced Cell Analysis
Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.
Fast Results
Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.
Simple 20 µl Cell-Based Assays
- Pre-qualified reagents
- Small 20 µl sample size
- Simple, image-based analysis
- Pre-defined instrument settings
- Assay-specific data templates
- Accurate, consistent results
Cell Types for Many Research Areas
Optimized for primary cell analysis
PBMCs
Stem Cells
Adipocytes
Neural Cells
Hepatocytes
Dendritic Cells
Epithelial Cells
Keratinocytes
Lymphocytes
Splenocytes
Monocytes
- Clinical Immunology: PBMCs
- Diabetes / Obesity: Adipocytes
- Immunotherapy: Leukocytes
- Microbiology: Yeast (Spectrum 10x)
- Oncology: Cell Lines
- Regenerative Medicine: Stem Cells
- Toxicology: Hepatocytes
- Transplantation: Nucleated Cells
- Vaccine Development: Splenocytes
Advantages of Cellometer Image Cytometry
Cell Imaging
- Visually check cell morphology
- Ensure only cells of interest are counted
- Archive and re-analyze cell images
- Export images for publication
Proprietary Pattern-Recognition Software
- Count individual cells in clusters
- Count irregular-shaped cells
- Count cells based on size
- Eliminate debris from cell counts
Non-Fluidic Platform
- Disposable counting chambers – no washing
- Compatible with fragile cells
- Maintenance-free
- Robust optics modules and LED light sources
IQ/OQ Validation and GMP/GLP Accessories
- Installation Qualification reagents/protocol
- Operational Qualification reagents/protocol
- On-site IQ or OQ Performance
- GMP/GLP Software Module
Accurate Cell Counting
Bright Field
Stained AO+ Cells
Counted AO+ Cells
- Automatic identification of Acridine Orange (AO) stained cells (shown above)
- Image and count up to 2×107 cells/mL
- Image acquisition and analysis:
30 seconds/sample for a 3-channel assay - Versatile platform – use for routine cell counting and complex cell-based assays
Export to FCS Express* for Flow-Like Data
Counted PI+ Cells
Contour Population Plot
- Export image data into flow cytometry software: FCS express
- Simple workflow: No fluid- stream, No PMT voltages, No forward/side scatter
- Easily perform data analysis using pre-designed templates
- Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot
Propidium Iodide (PI) Cell Cycle
* FCS Express Flow Cytometry software is a product of De Novo Software and is included with Cellometer Spectrum
| Cell Population | % of Gated Cells | Concentration (10^6 cells/mL) |
|---|---|---|
| Total
Sub G1 G0/G1 S G2/M |
100
3.8 61.9 15.3 19 |
3.18
0.12 1.97 0.49 0.60 |
Histogram of PE CD3+ Jurkat cells
CD3 Bright Field
CD3 PE
Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.
Bright Field
GFP/RFP Positive Cells
Proven Results
- Determine cell viability, for cell lines or primary samples, using AO/PI in seconds
- Accurately measure cell samples with varying viability (0 – 100%)
Bright Field
Bright Field
Stained AO+ Cells
Combined Counted Cells
User-Changeable Fluorescence Optics Modules*
Cellometer assays use specific optic modules for maximum performance and discrimination between fluorescence channels. Example filter modules are listed below. Additional filter modules available upon request. Please check our website for the most recent modules available.
| Optics Module | Fluorophores | Nucleic Acid Stains | |
|---|---|---|---|
| S1-534-470
Ex: 470nm (452-498nm) Em: 534nm (510-560nm) |
GFP
Calcein FITC AlexaFluor® 488 |
AO (acridine orange)
SYTO®9, SYTO®13 SYTOX®Green SYTO®BC |
|
| S1-605-527
Ex: 527nm (498-550nm) Em: 605nm (570-638nm) |
AlexaFluor® 546
AlexaFluor® 555 Cy3® PE (R-phycoerythrin) RFP |
PI (propidium iodide)
EB (ethidium bromide) SYTOX® Orange |
|
| S1-655-527
Ex: 527nm (525-558nm) Em: 655nm (642-665nm) |
RFP
7-AAD Nile Red |
PI (propidium iodide)
EB (ethidium bromide) |
|