//Disposable Hemacytometer
Disposable Hemacytometer2018-06-26T18:21:05+00:00

Cell Counting Methods

Learn how to count cells manually or move toward automating your workflow.

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Manual Hemacytometer

How do I count cells with a manual hemacytometer?

Order your calculation notepad

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Nexcelom Disposable Hemacytometer

How do I count cells with a Cellometer disposable hemacytometer?

How Cellometer hemacytometer is different

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Cellometer Automated Cell Counter

How do I automate my cell counting?

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How do I Count Cells with a Manual Hemacytometer?

Manual Cell Counting with Manual Hemacytometer

Step 1. Prepare the Hemacytometer

Clean hemacytometer and glass cover slip with 70% EtOH.


Step 2. Prepare Sample & Load Hemacytometer

Pipette 10 microliters of sample into the manual hemacytometer.


Step 3. Manually Count Cells in Sample

a. Place the hemacytometer under a microscope with a typical magnification of 100.
b. Focus both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares


Step 4. Cell Calculations & Disposal of Hemacytometer

a. Multiply the dilution factor by the total number of cells, divide by the # of corner squares counted, and multiply by 10E4 to obtain cell concentation (cells/ml).
b. Clean hemacytometer and glass cover slip with 70% EtOH.


Order your calculation notepad

How do I Count Cells with a Nexcelom Disposable Hemacytometer?

Manual Cell Counting with a Nexcelom Disposable Hemacytometer

Step 1. Prepare Sample & Load Hemacytometer

Pipette 20 microliters of sample into the Nexcelom disposable hemacytometer


Step 2. Manually Count Cells in Sample

a. Place the disposable hemacytometer under a microscope with a typical magnification of 100.
b. Focused both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares


Step 3. Cell Calculations & Disposal of Hemacytometer

a. Multiply the dilution factor by the total number of cells and divide by the sample volume.
b. Dispose the hemacytometer.


What Makes Our Hemacytometer Different?

  • All-in-one design
  • No cleaning or assembly of parts
  • Two counting chambers per slide
  • No washing biohazard
  • Easily disposed of after use
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Details of the Nexcelom Disposable Hemacytometer

Disposable Hemacytometer with two cell counting chambers per slide for replicates

Nexcelom Bioscience’s Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.

Cell counting grid pattern is the Improved Neubauer, as in a common Hemacytometer

The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.

Nexcelom CP2 Disposable Hemacytometer

Catalog # Description Unit
CP2-001 Plastic cell counting chamber slides with 2 chambers per slide containing a hemacytometer grid on the slide Box of 25 slides for 50 counts
CP2-002 Plastic cell counting chamber slides with 2 chambers per slide containing a hemacytometer grid on the slide Box of 100 slides for 200 counts

Ready to Automate Your Cell Counting?

Automated Cell Counting using Cellometer Automated Cell Counters

  • Improve accuracy & increase throughput
  • Automatically calculate concentration & viability
  • Save images & cell size histograms
  • GFP transfection rates & fluorescence cell counts

Pipette 20 µL

Insert Slide

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