Nexcelom Bioscience

978-327-5340

Measure Apoptosis in 3D Tumor Spheroids
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Monitor Apoptosis in 3D Tumor Spheroids

  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive bright field imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay

Introduction

The Celigo imaging cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

Celigo imaging process

Measure an Increase in Caspase 3/7 Activity in HCT116 3D Tumor Spheroids

increase in Caspase 3/7 activity in HCT116 3D tumor spheroids

HCT116R2 and HCT116 spheroids were stained with caspase 3/7 reagent and imaged. The above images present a merge of bright field and caspase 3/7 (green) images.

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