3D cultures were treated with multiple drugs (17-AAG, Paclitaxel, TMZ or Doxorubicin) and stained for viability after 21 days.
Live / dead stains used were calcein AM (green), Propidium Iodide (red) to measure live and dead cells on days 4, 7, 10, 14, and 17. Plates containing spheroids were imaged on Celigo imaging cytometer.
- 17-AAG decreased sphere size and caused the most significant cell death
- Paclitaxel decreased sphere size but maintained a significant number of live cells
- Temozolomide caused no significant decrease in sphere size and did not cause cell death
- Doxorubicin decreased sphere size and caused significant cell death