Nexcelom Bioscience

978-327-5340

Measure the Cell Invasion from a Spheroid into Matrigel

  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive bright field imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay

Introduction

The Celigo imaging cytometer has been developed to fully automate imaging and analysis of 3D spheroids. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

Celigo imaging process

Reduced Cell Invasion from a U-87 MG 3D Spheroid into Matrigel in the Presence of 17-AAG

Experimental Setup

invasion into Matrigel setup
  • Day 0-4: form spheroids
  • Day 4: add Matrigel to provide a semi-solid gel like matrix
  • Day 4-7: use Celigo imaging cytometer to determine the area occupied by individual cells or cell clusters

Bright Field Spheroid Images

invasion into matrigel

U-87 MG + 17-AAG

invasion into matrigel data

Measure Cell Invasion from 3D Spheroid into Matrigel

Pre-Matrigel

Bright Field
Spheroid

Bright Field Spheroid image

Bright Field
Identified Spheroid

Bright Field Identified Spheroid image

Percent confluence and represented fill view as seen in the Celigo software

Percent confluence and represented fill view

Measure Cell Invasion from 3D Spheroid into Matrigel

48 hr after the addition of Matrigel

Bright Field
Spheroid

Bright Field Spheroid image

Bright Field
Identified Spheroid

Bright Field Identified Spheroid image

Percent confluence and represented fill view as seen in the Celigo software

Percent confluence and represented fill view post Matrigel
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