Nexcelom Bioscience
Image Cytometer - Fluorescent concentration and viability analysis of yeast, platelets and other small cells

The X1 and X2 are PC-based cell imagers for automated 10x bright field and either single channel (X1) or dual channel (X2) fluorescent image capture and analysis. Both instruments accurately perform size-based cell counting, viability, intensity, and population analysis and achieve those measurements in less than 60 seconds. The instruments' advanced image analysis software omits debris and is optimal for yeast, platelets and other small cells. The X1 and X2 are ideal for measuring yeast concentration and viability in brewing fermentations. In addition, lager and ale yeast vitality can be measured using the X2.

Automated Fluorescent Cell Counting in <60 Seconds

Cell Counting Made Simple

Cell Counting Made Simple
Fluorescent Analysis

The Cellometer X2 utilizes bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

5x Faster than Manual Counting 5x Faster than Manual Counting: Load sample, view image, count cells, and obtain results in < 60 seconds

The Cellometer X2 Allows Hemacytometer Users to:

  • Increase throughput
  • Improve consistency
  • Ensure all data is correctly captured
  • Count difficult cells (clumpy, irregular-shaped)
  • Eliminate judgment errors, miscounts, and user-to-user variability

Fluorescent Analysis

Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence when imaged with the Cellometer X2.

Because mature mammalian red blood cells don't contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive nucleated cells are easily counted. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.

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Fluorescent Viability Determination

Use of a fluorescent dye, such as propidium iodide (PI), is highly recommended for accurate viability analysis of cell samples containing debris.

Propidium iodide (PI) is a nuclear staining (nucleic acid binding) dye that enters dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence.

Because the Cellometer X2 can count dead cells in the red fluorescence channel, there is no interference from debris and live cells.

With propidium iodide (PI), dead cells are clearly visible in the red fluorescence channel.

Fluorescent Viability Determination

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1-Step Yeast Concentration & Viability Analysis using Cellometer X2

Bright field and Fluorescent Imaging

1-Step Yeast Concentration and Viability
Bright field and fluorescent images of yeast cells. Cells appearing in both the bright field and fluorescent image are dead.

The Cellometer X2 Image Cytometer is specifically optimized for simple, 1-step determination of yeast concentration and viability. The Cellometer X2 is ideal for research laboratories and small and large breweries looking to automate their fermentation monitoring. Performance of the Cellometer X2 has been proven in the largest breweries in the U.S.

Bright field images can be viewed to confirm cell morphology. Counted bright field images show counted cells within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both bright field and fluorescent images from each sample makes 1-step concentration & viability determination possible. Read more about yeast viability.

Yeast Concentration Measurement by Bright Field Analysis

Single Cell Count

Yeast single cell count

De-clustering of Yeast Cells

Decluster budding yeast cells

Chain-Forming Cell Count

Yeast Chain-Forming Cell Count

Yeast Concentration & Viability Measurement by Bright Field & Fluorescence

Viability Measurement Using Propidium Iodide (PI)

Yeast viability by PI

Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.

Viability Measurement by Oxonol

Yeast viability by Oxonol

Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.

Yeast Concentration Measurement by Dual Fluorescence Detection

Yeast Viability by Dual-Fluorescence

Yeast Viability by Dual-Fluorescence

Yeast samples are stained 1-to-1 with a mixture of acridine orange (AO) and propidium iodide (PI) dual-fluorescence stain. Yeast concentration and viability are obtained immediately after staining using Cellometer X2. Live yeast cells fluoresce green and dead cells fluoresce red.

Yeast Vitality by Fluorescent Enzymatic Stain

Yeast Vitality by fluorescent enzymatic stain

Yeast samples are stained 1-to-1 with Carboxyfluorescein-AM fluorescent enzymatic stain for 45 minutes and then analyzed for vitality using Cellometer X2. Bright field images are used for total cell count and fluorescent images are used to measure the active yeast cells.

Yeast Cell Cycle Analysis Using Propidium Iodide (PI)

Yeast Cell Cycle Analysis

Standard baker's yeast stained with the Cell Cycle Staining Kit from Nexcelom Bioscience are incubated for 60 minutes before using Cellometer X2 to analyze the cell cycle. The plot shows the yeast population that is actively dividing. Their higher DNA content is measured using PI.

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Automated Platelet Counting in Whole Blood

Automated Platelet counting in whole blood

Fluorescence-Based Platelet Concentration. Measurement A blood sample stained using the Calcein AM Vitality / Viability Kit from Nexcelom Bioscience is incubated for 20 minutes. Both platelets and white blood cells produce green fluorescence. Cell size gating is applied to exclusively count platelets.

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Exclusion of Debris & Size-Based Counting

Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.

  • Cell size parameters can be modified to optimize exclusion of debris from results and enhance the accuracy of counting for a wide range of cell sizes.
  • A fluorescent nuclear dye, such as acridine orange, can be used to more easily identify nucleated cells in samples containing debris.
Counted cell image excludes debris
Cell Size Histogram

The Cellometer X2 Image Cytometer automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.

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View, Print, and Save Cell Images and Data Tables

counted cell images of mouse splenocytes
Bright field and fluorescent counted images of mouse splenocytes stained with acridine orange.

View bright field images to check cell morphology.

View fluorescent counted images to confirm exclusion of debris or unwanted cell types and correct counting of cells within clumps.

Export Data

Easily Export to Excel for additional data formatting or sharing. Images and data tables can be pasted into PowerPoint presentations or submitted for publication.

Save Data

Easily Save data and images to a network or local computer.

Print

Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images.

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Cellometer X1 vs. X2

Cellometer Yeast Analysis
X1
X2
Bright field imaging mode
X
X
Single fluorescence imaging mode
X
X
Dual fluorescence imaging mode
X
Viability using PI
X
X
Concentration & viability using AOPI
X
Vitality using CFDA-AM
X
Yeast cell cycle
X
X
Cellometer software for analysis of clumpy and
irregular-shaped cells
X
X
Mean diameter and cell size distribution
X
X
Cell type wizard for creating new cell type parameters
X
X

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Counting Chambers: No Washing or Contamination

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette.

The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.

Cell Counting Chamber
The disposable Cellometer Cell Counting Chambers offer several key advantages:
  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume
  • Most affordable automated counting consumables

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Dedicated On-line and On-site Applications Support

Nexcelom Technical Support Specialists

Experienced Nexcelom Technical Support Specialists are available from 9am to 5pm EST for phone and on-line support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer X2 Cell Counter

Help

The help button at the bottom right of the Cellometer X2 software screen gives users instant access to:

  • Software features and instructions
  • On-line tutorials and training videos
  • Submission of a Support Ticket

Submit a Support Ticket

Clicking "Submit a Support Ticket" launches a convenient on-line form that is submitted directly to Nexcelom Technical Support

  • X2 instrument information fields are automatically populated
  • Images are easily attached for troubleshooting and applications support
  • A Nexcelom Specialist can assume remote control of the Cellometer X2 Image Cytometer for troubleshooting and training

Nexcelom Field-based Applications Specialists

Nexcelom Field-based Applications Specialists are also available for:

  • On-site demonstrations
  • Training
  • Troubleshooting
  • Technical Seminars
All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image-Based Cytometry

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