////Tumorsphere Formation & Clonogenic Survival
Tumorsphere Formation & Clonogenic Survival2018-06-11T12:58:56+00:00

Tumorsphere Formation & Clonogenic Survival

Perform tumorsphere formation and clonogenic assays to quantify size and number of formed tumorspheres.

  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive bright field imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay
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Celigo Product Guide
Applications Collection
WP: Breast Cancer Drugs

Introduction

The Celigo imaging cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

automate imaging and analysis of tumorspheres

Image and Quantify Tumorsphere Formation and Clonogenic Survival Assays

Celigo software imaging and quantification of tumorsphere

Celigo software displays a plate map, image view of the whole well, and numerical data for the counted spheres. The green fill view indicates counted spheres.

Formation and Counting of Tumorspheres from Various Cell Lines

tumorsphere formation MDA-MB-436

Whole-well image and individually counted MDA-MB-436 formed tumorspheres (outlined in green).

tumorsphere formation SKBR3

Whole-well image and individually counted SK-BR-3 formed tumorspheres (outlined in green).

tumorsphere formation MCF7

Whole well image and individually counted MCF-7 formed tumorspheres (outlined in green).

Quantification of Methylene Blue-Stained Clonogenic Colonies in 6-well Plates

MDA-MB-468 colonies stained with methylene blue

MDA-MB-468 colonies were stained with methylene blue 18 days following siRNA knockdown. The plate was imaged on Celigo at 3 um/px.

Whole-well image of the control sample

whole-well image MDA-MB468 cells

Whole-well image and counted colonies of siRNA treated sample

whole-well image counted colonies siRNA
MDA-MB468 cell data following siRNA knockdown

Colony number of MDA MB468 cells following siRNA knockdown