////Single Tumor Sphere Identification
Single Tumor Sphere Identification2018-06-11T13:29:08+00:00

Single Tumor Sphere Identification

Monitor the Tumor Sphere Formation from a Single Cell to a Single Sphere in Microwell Plates

Single Cell to Single Tumor Sphere

A single cell is seeded per well and the formation of a tumor sphere is monitored over the next 15 days using the Celigo and the tumor sphere application. Celigo captures whole-well images on day 0 to document the presence of 1 cell per well. This data is used to verify that the formation of the sphere originated from a single cell.

Quantification of tumor sphere size

On day 15 the entire 96-well plate is imaged and the tumor sphere size is automatically analyzed. The size distribution graph on the right shows a variation of tumor sphere sizes, (100 – 300 microns) that arose from single cell clones seeded on day one. The Celigo-captured images (below) show the great variation in formed tumor sphere size and morphology.

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Tumor sphere formation

Tumor sphere formation

Tumor sphere generated from single cell size distribution

Tumor sphere generated from single cell size distribution